Construct: ORF ccsbBroadEn_15231
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018956.1_s300c1, BRDN0000458252
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- LRRK2 (120892)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 120892 | LRRK2 | leucine rich repeat kinase 2 | NM_198578.4 | 97.8% | 1.4% | (many diffs) |
| 2 | human | 120892 | LRRK2 | leucine rich repeat kinase 2 | XM_005268629.4 | 97.6% | 1.5% | (many diffs) |
| 3 | human | 120892 | LRRK2 | leucine rich repeat kinase 2 | XM_011537877.3 | 96.9% | 1.5% | (many diffs) |
| 4 | human | 120892 | LRRK2 | leucine rich repeat kinase 2 | XM_024448833.1 | 83.9% | 1.4% | (many diffs) |
| 5 | human | 120892 | LRRK2 | leucine rich repeat kinase 2 | XR_001748574.2 | 64.5% | (many diffs) | |
| 6 | human | 120892 | LRRK2 | leucine rich repeat kinase 2 | XM_011537881.3 | 61.9% | 2.3% | (many diffs) |
| 7 | human | 120892 | LRRK2 | leucine rich repeat kinase 2 | XM_017018787.1 | 59% | 1.4% | (many diffs) |
| 8 | human | 120892 | LRRK2 | leucine rich repeat kinase 2 | XM_017018786.2 | 58.5% | 2.4% | (many diffs) |
| 9 | human | 120892 | LRRK2 | leucine rich repeat kinase 2 | XM_017018788.2 | 50.2% | .8% | (many diffs) |
| 10 | human | 120892 | LRRK2 | leucine rich repeat kinase 2 | XM_011537882.3 | 48.6% | 2.9% | (many diffs) |
| 11 | human | 120892 | LRRK2 | leucine rich repeat kinase 2 | XM_017018789.2 | 48.4% | 2.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 99
- ORF end:
- 420
- ORF length:
- 321
- Sequence:
-
1 CCATGATTTT ATTTTGACTG ATAGTGACCT GTTCGTTGCA ACAAATTGAT GAGCAATGCT 61 TTTTTATAAT GCCAACTTTG TACAAAAAAG TTGGCACCAT GGCTAGTGGC AGCTGTCAGG 121 GGTGCGAAGA GGACGAGNAA ACTTCTGAAG AAGTTGATAG TCAGGCTGAA CAATGTCCAG 181 GAAGGAAAAC AGATAGAAAC GCTGGTCCAA ATCCTGGAGG ATCTGCTGGT GTTCACGTAC 241 TCCGAGCACG CCTCCAAGTT ATTTCAAGGC AAAAATATCC ATGTGCCTCT GTTGATCGTC 301 TTGGACTCCT ATATGAGAGT CGCGAGTGTG CAGCAGGTGG GTTGGTCACT TCTGTGCAAA 361 TTAATAGAAG TCTGTCCAGG TACAATGCAA AGCTTAATGG GACCCCAGGA TGTTGGAAAT 421 GATTGGGAAG TCCTTGGTGT TCACCAATTG ATTCTTAAAA TGCTAACAGT TCATAATGCC 481 AGTGTAAACT TGTCAGTGAT TGGACTGAAG ACCTTAGATC TCCTCCTAAC TTCAGGTAAA 541 ATCACCTTGC TGATATTGGA TGAAGAAAGT GATATTTTCA TGTTAATTTT TGATGCCATG 601 CACTCATTTC CAGCCAATGA TGAAGTCCAG AAACTTGGAT GCAAAGCTTT ACATGTGCTG 661 TTTGAGAGAG TCTCAGAGGA GCAACTGACT GAATTTGTTG AGAACAAAGA TTATATGATA 721 TTGTTAAGTG CGTTAACAAA TTTTAAAGAT GAAGAGGAAA TTGTGCTTCA TGTGCTGCAT 781 TGTTTACATT CCCTAGCGAT TCCTTAGATA CAATCTTGCT TGACCGTTTG GGACATCAAT 841 NCTTCCACAT GAANNTGCAA AATTTAGAAA AACACATTGA AGTGAGAAAA GAATTAGCTG 901 AAAAAATGAG ACGAACATCT GTTGAGTTGC CAACTTTCTT GTACAAAGTT GGCATTATAA 961 GAAAGCATTG CTTATCAATT TGTTGCAACG AACAGGTCAC TATCAGTCAA AATAAAATCA 1021 TTATTGCCAT CCAGCTAGCT CAGTGctcag ctgtttggcc ctcctcactg agactatttt 1081 cttaaatcaa gatttagagg aaaagaatga gaatcaagag aatgatgatg agggggaaga 1141 agataaattg ttttggctgg aagcctgtta caaagcatta acgtggcata gaaagaacaa 1201 gcacgtgcag gaggccgcat gctgggcact aaataatctc cttatgtacc aaaacagttt 1261 acatgagaag attggagatg aagatggcca tttcccagct catagggaag tgatgctctc 1321 catgctgatg cattcttcat caaaggaagt tttccaggca tctgcgaatg cattgtcaac 1381 tctcttagaa caaaatgtta atttcagaaa aatactgtta tcaaaaggaa tacacctgaa 1441 tgttttggag ttaatgcaga agcatataca ttctcctgaa gtggctgaaa gtggctgtaa 1501 aatgctaaat catctttttg aaggaagcaa cacttccctg gatataatgg cagcagtggt 1561 ccccaaaata ctaacagtta tgaaacgtca tgagacatca ttaccagtgc agctggaggc 1621 gcttcgagct attttacatt ttatagtgcc tggcatgcca gaagaatcca gggaggatac 1681 agaatttcat cataagctaa atatggttaa aaaacagtgt ttcaagaatg atattcacaa 1741 actggtccta gcagctttga acaggttcat tggaaatcct gggattcaga aatgtggatt 1801 aaaagtaatt tcttctattg tacattttcc tgatgcatta gagatgttat ccctggaagg 1861 tgctatggat tcagtgcttc acacactgca gatgtatcca gatgaccaag aaattcagtg 1921 tctgggttta agtcttatag gatacttgat tacaaagaag aatgtgttca taggaactgg 1981 acatctgctg gcaaaaattc tggtttccag cttataccga tttaaggatg ttgctgaaat 2041 acagactaaa ggatttcaga caatcttagc aatcctcaaa ttgtcagcat ctttttctaa 2101 gctgctggtg catcattcat ttgacttagt aatattccat caaatgtctt ccaatatcat 2161 ggaacaaaag gatcaacagt ttctaaacct ctgttgcaag tgttttgcaa aagtagctat 2221 ggatgattac ttaaaaaatg tgatgctaga gagagcgtgt gatcagaata acagcatcat 2281 ggttgaatgc ttgcttctat tgggagcaga tgccaatcaa gcaaaggagg gatcttcttt 2341 aatttgtcag gtatgtgaga aagagagcag tcccaaattg gtggaactct tactgaatag 2401 tggatctcgt gaacaagatg tacgaaaagc gttgacgata agcattggga aaggtgacag 2461 ccagatcatc agcttgctct taaggaggct ggccctggat gtggccaaca atagcatttg 2521 ccttggagga ttttgtatag gaaaagttga accttcttgg cttggtcctt tatttccaga 2581 taagacttct aatttaagga aacaaacaaa tatagcatct acactagcaa gaatggtgat 2641 cagatatcag atgaaaagtg ctgtggaaga aggaacagcc tcaggcagcg atggaaattt 2701 ttctgaagat gtgctgtcta aatttgatga atggaccttt attcctgact cttctatgga 2761 cagtgtgttt gctcaaagtg atgacctgga tagtgaagga agtgaaggct catttcttgt 2821 gaaaaagaaa tctaattcaa ttagtgtagg agaattttac cgagatgccg tattacagcg 2881 ttgctcacca aatttgcaaa gacattccaa ttccttgggg cccatttttg atcatgaaga 2941 tttactgaag cgaaaaagaa aaatattatc ttcagatgat tcactcaggt catcaaaact 3001 tcaatcccat atgaggcatt cagacagcat ttcttctctg gcttctgaga gagaatatat 3061 tacatcacta gacctttcag caaatgaact aagagatatt gatgccctaa gccagaaatg 3121 ctgtataagt gttcatttgg agcatcttga aaagctggag cttcaccaga atgcactcac 3181 gagctttcca caacagctat gtgaaactct gaagagtttg acacatttgg acttgcacag 3241 taataaattt acatcatttc cttcttattt gttgaaaatg agttgtattg ctaatcttga 3301 tgtctctcga aatgacattg gaccctcagt ggttttagat cctacagtga aatgtccaac 3361 tctgaaacag tttaacctgt catataacca gctgtctttt gtacctgaga acctcactga 3421 tgtggtagag aaactggagc agctcatttt agaaggaaat aaaatatcag ggatatgctc 3481 ccccttgaga ctgaaggaac tgaagatttt aaaccttagt aagaaccaca tttcatccct 3541 atcagagaac tttcttgagg cttgtcctaa agtggagagt ttcagtgcca gaatgaattt 3601 tcttgctgct atgcctttct tgcctccttc tatgacaatc ctaaaattat ctcagaacaa 3661 attttcctgt attccagaag caattttaaa tcttccacac ttgcggtctt tagatatgag 3721 cagcaatgat attcagtacc taccaggtcc cgcacactgg aaatctttga acttaaggga 3781 actcttattt agccataatc agatcagcat cttggacttg agtgaaaaag catatttatg 3841 gtctagagta gagaaactgc atctttctca caataaactg aaagagattc ctcctgagat 3901 tggctgtctt gaaaatctga catctctgga tgtcagttac aacttggaac taagatcctt 3961 tcccaatgaa atggggaaat taagcaaaat atgggatctt cctttggatg aactgcatct 4021 taactttgat tttaaacata taggatgtaa agccaaagac atcataaggt ttcttcaaca 4081 gcgattaaaa aaggctgtgc cttataaccg aatgaaactt atgattgtgg gaaatactgg 4141 gagtggtaaa accaccttat tgcagcaatt aatgaaaacc aagaaatcag atcttggaat 4201 gcaaagtgcc acagttggca tagatgtgaa agactggcct atccaaataa gagacaaaag 4261 aaagagagat ctcgtcctaa atgtgtggga ttttgcaggt cgtgaggaat tctatagtac 4321 tcatccccat tttatgacgc agcgagcatt gtaccttgct gtctatgacc tcagcaaggg 4381 acaggctgaa gttgatgcca tgaagccttg gctcttcaat ataaaggctc gcgcttcttc 4441 ttcccctgtg attctcgttg gcacacattt ggatgtttct gatgagaagc aacgcaaagc 4501 ctgcatgagt aaaatcacca aggaactcct gaataagcga gggttccctg ccatacgaga 4561 ttaccacttt gtgaatgcca ccgaggaatc tgatgctttg gcaaaacttc ggaaaaccat 4621 cataaacgag agccttaatt tcaagatccg agatcagctt gttgttggac agctgattcc 4681 agactgctat gtagaacttg aaaaaatcat tttatcggag cgtaaaaatg tgccaattga 4741 atttcccgta attgaccgga aacgattatt acaactagtg agagaaaatc agctgcagtt 4801 agatgaaaat gagcttcctc acgcagttca ctttctaaat gaatcaggag tccttcttca 4861 ttttcaagac ccagcactgc agttaagtga cttgtacttt gtggaaccca agtggctttg 4921 taaaatcatg gcacagattt tgacagtgaa agtggaaggt tgtccaaaac accctaaggg 4981 aattatttcg cgtagagatg tggaaaaatt tctttcaaag aaaaggaaat ttccaaagaa 5041 ctacatgaca cagtatttta agctcctaga aaaattccag attgctttgc caataggaga 5101 agaatatttg ctggttccaa gcagtttgtc tgaccacagg cctgtgatag agcttcccca 5161 ttgtgagaac tctgaaatta tcatccgact atatgaaatg ccttattttc caatgggatt 5221 ttggtcaaga ttaatcaatc gattacttga gatttcacct tacatgcttt cagggagaga 5281 acgagcactt cgcccaaaca gaatgtattg gcgacaaggc atttacttaa attggtctcc 5341 tgaagcttat tgtctggtag gatctgaagt cttagacaat catccagaga gtttcttaaa 5401 aattacagtt ccttcttgta gaaaaggctg tattcttttg ggccaagttg tggaccacat 5461 tgattctctc atggaagaat ggtttcctgg gttgctggag attgatattt gtggtgaagg 5521 agaaactctg ttgaagaaat gggcattata tagttttaat gatggcgaag aacatcaaaa 5581 aatcttactt gatgacttga tgaagaaagc agaggaagga gatctcttag taaatccaga 5641 tcaaccaagg ctcaccattc caatatctca gattgcccct gacttgattt tggctgacct 5701 gcctagaaat attatgttga ataatgatga gttggaattt gaacaagctc cagagtttct 5761 cctaggtgat ggcagttttg gatcagttta ccgagcagcc tatgaaggag aagaagtggc 5821 tgtgaagatt tttaataaac atacatcact caggctgtta agacaagagc ttgtggtgct 5881 ttgccacctc caccacccca gtttgatatc tttgctggca gctgggattc gtccccggat 5941 gttggtgatg gagttagcct ccaagggttc cttggatcgc ctgcttcagc aggacaaagc 6001 cagcctcact agaaccctac agcacaggat tgcactccac gtagctgatg gtttgagata 6061 cctccactca gccatgatta tataccgaga cctgaaaccc cacaatgtgc tgcttttcac 6121 actgtatccc aatgctgcca tcattgcaaa gattgctgac tacggcattg ctcagtactg 6181 ctgtagaatg gggataaaaa catcagaggg cacaccaggg tttcgtgcac ctgaagttgc 6241 cagaggaaat gtcatttata accaacaggc tgatgtttat tcatttggtt tactactcta 6301 tgacattttg acaactggag gtagaatagt agagggtttg aagtttccaa atgagtttga 6361 tgaattagaa atacaaggaa aattacctga tccagttaaa gaatatggtt gtgccccatg 6421 gcctatggtt gaaaaattaa ttaaacagtg tttgaaagaa aatcctcaag aaaggcctac 6481 ttctgcccag gtctttgaca ttttgaattc agctgaatta gtctgtctga cgagacgcat 6541 tttattacct aaaaacgtaa ttgttgaatg catggttgct acacatcaca acagcaggaa 6601 tgcaagcatt tggctgggct gtgggcacac cgacagagga cagctctcat ttcttgactt 6661 aaatactgaa ggatacactt ctgaggaagt tgctgatagt agaatattgt gcttagcctt 6721 ggtgcatctt cctgttgaaa aggaaagctg gattgtgtct gggacacagt ctggtactct 6781 cctggtcatc aataccgaag atgggaaaaa gagacatacc ctagaaaaga tgactgattc 6841 tgtcacttgt ttgtattgca attccttttc caagcaaagc aaacaaaaaa attttctttt 6901 ggttggaacc gctgatggca agttagcaat ttttgaagat aagactgtta agcttaaagg 6961 agctgctcct ttgaagatac taaatatagg aaatgtcagt actccattga tgtgtttgag 7021 tgaatccaca aattcaacgg aaagaaatgt aatgtgggga ggatgtggca caaagatttt 7081 ctccttttct aatgatttca ccattcagaa actcattgag acaagaacaa gccaactgtt 7141 ttcttatgca gctttcagtg attccaacat cataacagtg gtggtagaca ctgctctcta 7201 tattgctaag caaaatagcc ctgttgtgga agtgtgggat aagaaaactg aaaaactctg 7261 tggactaata gactgcgtgc actttttaag ggaggtaacg gtaaaagaaa acaaggaatc 7321 aaaacacaaa atgtcttatt ctgggagagt gaaaaccctc tgccttcaga agaacactgc 7381 tctttggata ggaactggag gaggccatat tttactcctg gatctttcaa ctcgtcgact 7441 tatacgtgta atttacaact tttgtaattc ggtcagagtc atgatgacag cacagctagg 7501 aagccttaaa aatgtcatgc tggtattggg ctacaaccgg aaaaatactg aaggtacaca 7561 aaagcagaaa gagatacaat cttgcttgac cgtttgggac atcaatcttc cacatgaagt 7621 gcaaaattta gaaaaacaca ttgaagtgag aaaagaatta gctgaaaaaa tgagacgaac 7681 atctgttgag ttgccaactt tcttgtacaa agttggcatt ataagaaagc attgcttatc 7741 aatttgttgc aacgaac