Construct: ORF ccsbBroadEn_15251
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018923.1_s300c1, BRDN0000458219
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- DGKK (139189)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 139189 | DGKK | diacylglycerol kinase kappa | NM_001013742.4 | 99.5% | 18.5% | (many diffs) |
| 2 | human | 139189 | DGKK | diacylglycerol kinase kappa | XM_017029268.2 | 97.2% | 19% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 97
- ORF end:
- 910
- ORF length:
- 813
- Sequence:
-
1 AAGGATTTAT TTTGACTGAT AGTGACCTGT TCGTTGCAAC AAATTGATGA GCAATGCTTT 61 TTTATAATGC CAACTTTGTA CAAAAAAGTT GGCACCATGG ACCGCGGAGC TGCCGCAGCC 121 CAGGGCACTG CCCCGCCTCA GGATGGAGAG CAGCCCGCTG AGTCTCCAGA GCCTCCGCCG 181 CCTTGGCCGC CGCCGCCACC ACCACCGGCT CCGCCGCCGG CTCCGCCGCT GCTCTCCGAG 241 GCTTCGCCAG AACCCATACC AGAGCCCTGT CCAGAGCTTG CTCCAGGTCC CTGTCCAGAG 301 GCGACCTCAG AATCAGCCAC AGAACTGTAC ACAGAACCGA CCCCAGAACC AGCCACAGAG 361 CCGGCCTCAG AACCGGCCCC AGAACCTGCC ACAGAGCCGG CCCCAGAACC TGCCACAGAG 421 CCGGCCCCAG AACCGGCCCC AGAACCTGCC ACAGAGTCTG CCCCAGAGCC GACTCCAGAA 481 CCTGCCCTAG AGTCGGTCCC AGAGCCGGCC CCAGAGCTGA CTCCAGAAGT TGCCCCAGAG 541 CTGGCCCCAG AGCCGACCCC AGAACCTGTG ACAGAGCTGG CCCCAGAGTT CTGCCCTGAG 601 GCGGCTCCAG AGTTCCGTCC AAGTCCAGCA CCATGTCTAT TGCAATGTCC GGTGGACACT 661 CGAGAGAGAG GTCTAAAGAC CTCGCCATCG CCATCGCCAT CGCCATCGCC CAGAACGCCA 721 ATGTCGTGGT CCAGAATAAA GAAAATATTG AAGGAAGGAC CTATGCTGAA GAACTGTAAC 781 TCTTTCAGAG ATGGAAGCTT AGATATTTTC TGGTTCAAGG ACAGAAGCTC TACTTTGCAC 841 ACCATCCCGC GTTTGCACAC TTTGAAACGA TTGATCTGTC TCAAGCCACT GGTGGCAGAA 901 AGGCAGCTGT AGAAACCCTT TGGCCAACAG GATTGTTgtt attacaccac aacgaaaaat 961 cactctggct gcacccaacc ggaaagacat ggaagaatgg attaacatca taaaaaccat 1021 ccaacaggga gaaatttata agatacctgc agcagaaaac aacccttttc ttgttggaat 1081 gcattgttgg tactccagtt acagccaccg gacccagcac tgcaatgttt gtcgagagag 1141 cattcctgcc ttatctagag atgccatcat ctgtgaagtg tgcaaagtga aatctcacag 1201 attgtgtgct ttgagagcaa gcaaagactg caagtggaat acattgtcta tcactgatga 1261 cctccttctg cctgcagatg aagtaaacat gccccatcaa tgggtagaag gaaacatgcc 1321 tgtcagctct cagtgtgcag tgtgtcatga gagctgtggc agttatcaaa gacttcaaga 1381 cttccgctgc ctgtggtgta attctacggt gcatgatgac tgtaggagac ggttttccaa 1441 ggaatgttgc ttcagaagcc atcgctcatc agtcattcct cccactgctc taagcgaccc 1501 caaaggcgat ggccaattag tagtatcttc agacttctgg aatcttgatt ggtcatcagc 1561 ctgttcatgt cccttgctca tcttcatcaa ctccaaaagt ggcgatcatc aggggatcgt 1621 cttcctccga aaattcaagc aataccttaa cccatctcaa gtgttcgact tattgaaggg 1681 tggacctgaa gcagggctgt ctatgttcaa gaactttgct cgctttcgca ttctggtttg 1741 tggtggagat ggcagcgtga gctgggtctt atctctgatt gatgcctttg gattacatga 1801 aaagtgtcag ttggcagtca tcccacttgg aaccggcaat gatctggctc gtgttctggg 1861 ctggggtgca ttctggaaca aaagcaagtc acctctggac atcctcaaca gagtggagca 1921 ggctagtgtg aggatcctag acagatggag tgtgatgatt cgtgagactc ccagacaaac 1981 cccgctgcta aaaggacagg ttgaaatgga tgtaccacga tttgaggctg ctgccatcca 2041 acacttagaa tctgcagcca ccgagttgaa caaaatcctg aaggccaagt accccacaga 2101 gatgatcatc gcaaccagat tcttgtgttc agctgtggaa gattttgtgg ttgatattgt 2161 aaaggcctgg ggtcagataa aacagaacaa cactgcaata gtgtctgtga ttttgaaaag 2221 tgacttaatg tatgataggc tcagtgtcct gatcgatgtc ctggctgagg aggcagcagc 2281 tacttctgct gaaaaaagtg ctacagaata tgcagacagc agcaaggcag ataggaagcc 2341 cttcattcct caaatagacc acatagccaa gtgcaagttg gagctggcta caaaggccca 2401 gagtctccag aaatccttga aactcatcat atttcaagtt gaacaagctc tggatgagga 2461 aagcagacag acaatatctg ttaagaactt tagttcaact ttcttcctgg aagatgaccc 2521 agaagatatt aaccagacaa gcccacgacg ccgttctcgt cgtggcactt tgtcttctat 2581 atcttctctc aaaagtgagg acctggacaa ccttaacttg gatcacttac attttacacc 2641 tgaatctata cgcttcaaag aaaaatgtgt catgaacaac tacttcggaa ttggactgga 2701 tgctaaaatt tctctggact tcaacaccag aagagatgaa cacccagggc aatacaatag 2761 ccgccttaag aacaagatgt ggtatggcct tctgggaacc aaagaacttt tgcagcgctc 2821 ttacaggaaa ctggaagaac gagtgcattt ggagtgtgat ggagaaacca tctccttgcc 2881 aaacctgcaa ggcattgtag tgctcaacat taccagctat gctggaggta tcaacttctg 2941 gggaagcaac acagcaacca cggaatatga ggctcctgca atcgatgatg ggaaactgga 3001 ggtggtggca atctttggtt ctgtgcagat ggcaatgtcc cgtatcatca acctgcatca 3061 tcatcgcatt gcccagtgcc atgaggtgat gataaccatt gatTGTGAAG AAGGTATCCC 3121 AGGGCAGGTG ATGGGGGAGG CTTGGATTCA GAGACCAGGC TTTATCAAAA ATAGATACAA 3181 GAACGCTGCC CAGATGCTGA CAAGAGATCG GGACTTTGAG AACTCAATGA AAATGTGGGA 3241 ATACAAGCAT ACTGAAATTC AAGCTGCCCC TCAACCCCAG CTGGACTTCC AGGACTCTCA 3301 AGAGAGCCTC TCTGACGAGG AGTATGCCCA GATGCAGCAC TTAGCTCGGC TTGCAGAAAA 3361 CCTCATCAGC AAACTTAATG ACCTGAGCAA GATCCACCAG CATGTGTCTG TCCTCATGGG 3421 TTCTGTGAAT GCCAGCGCTA ACATCCTGAA TAATATATTT TACGGCCAAG ACAGTGGCAA 3481 TGAGATGGGT GCAGCTTCCT GTATTCCCAT TGAAACTCTA AGCAGAAATG ATGCCGTAGA 3541 TGTTACATTT AGTCTTAAAG GTCTCTACGA TGACACCACA GCTTTCCTGG ATGAAAAGCT 3601 GCTGAGAAGT GCTGAGGATG AGACTGCACT ACAAAGCGCC CTGGATGCCA TGAATAAGGA 3661 GTTCAAAAAG CTATCTGAGA TTGACTGGAT GAATCCAATC TTTGTTCCAG AGGAAAAATC 3721 TTCGGACACT GACAGTAGAA GCCTCAGGCT GAAAATTAAG TTCCCCAAAT TGGGAAAGAA 3781 AAAGGTAGAA GAGGAACGCA AGCCTAAATC AGGCCAGAGT GTCCAGAGTT TTATTGGCAA 3841 TTTATGGCAC CGCAGACATC GTGAAGATGA AGCAGAGGGT GATGATCCTC TAACACCATC 3901 GAGATCTCAA CTGTTGCCAA CTTTCTTGTA CAAAGTTGGC ATTATAAGAA AGCATTGCTT 3961 ATCAATTTGT TGCAACGAAC AGGTCACTAT CAGTCAAAAT AAAATCATTA TTGCCATCCC 4021 AGCTTGATAT