Construct: ORF ccsbBroadEn_15253
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018960.1_s300c1, BRDN0000458256
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- MYO3B (140469)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 140469 | MYO3B | myosin IIIB | NM_138995.5 | 99.8% | 81.2% | (many diffs) |
| 2 | human | 140469 | MYO3B | myosin IIIB | XM_006712299.4 | 99.1% | 80.6% | (many diffs) |
| 3 | human | 140469 | MYO3B | myosin IIIB | NM_001083615.4 | 97.8% | 83.4% | (many diffs) |
| 4 | human | 140469 | MYO3B | myosin IIIB | XM_011510655.3 | 97.1% | 82.8% | (many diffs) |
| 5 | human | 140469 | MYO3B | myosin IIIB | XM_011510654.3 | 96.5% | 78.5% | (many diffs) |
| 6 | human | 140469 | MYO3B | myosin IIIB | XM_011510656.3 | 74.8% | 57.4% | (many diffs) |
| 7 | human | 140469 | MYO3B | myosin IIIB | XM_011510657.3 | 73.5% | 55.4% | (many diffs) |
| 8 | human | 140469 | MYO3B | myosin IIIB | XM_011510658.3 | 73.5% | 55.4% | (many diffs) |
| 9 | human | 140469 | MYO3B | myosin IIIB | XM_024452713.1 | 73.5% | 55.4% | (many diffs) |
| 10 | human | 140469 | MYO3B | myosin IIIB | NR_045683.2 | 64.3% | (many diffs) | |
| 11 | human | 140469 | MYO3B | myosin IIIB | NR_045682.2 | 63.4% | (many diffs) | |
| 12 | human | 140469 | MYO3B | myosin IIIB | NR_045684.2 | 63% | (many diffs) | |
| 13 | mouse | 329421 | Myo3b | myosin IIIB | XM_017319039.1 | 81.8% | 75.4% | (many diffs) |
| 14 | mouse | 329421 | Myo3b | myosin IIIB | NM_177376.4 | 81.6% | 75.3% | (many diffs) |
| 15 | mouse | 329421 | Myo3b | myosin IIIB | XM_006499745.3 | 80.8% | 74.4% | (many diffs) |
| 16 | mouse | 329421 | Myo3b | myosin IIIB | XM_017319037.1 | 80.8% | 74.4% | (many diffs) |
| 17 | mouse | 329421 | Myo3b | myosin IIIB | XM_017319038.1 | 80% | 73.7% | (many diffs) |
| 18 | mouse | 329421 | Myo3b | myosin IIIB | XM_017319040.1 | 79.5% | 72.9% | (many diffs) |
| 19 | mouse | 329421 | Myo3b | myosin IIIB | XM_006499750.3 | 78.7% | 71.9% | (many diffs) |
| 20 | mouse | 329421 | Myo3b | myosin IIIB | XM_017319041.1 | 75.9% | 79% | (many diffs) |
| 21 | mouse | 329421 | Myo3b | myosin IIIB | XM_017319042.1 | 75.2% | 79.3% | (many diffs) |
| 22 | mouse | 329421 | Myo3b | myosin IIIB | XM_017319043.1 | 75.1% | 79.6% | (many diffs) |
| 23 | mouse | 329421 | Myo3b | myosin IIIB | XM_017319044.1 | 74.1% | 82.7% | (many diffs) |
| 24 | mouse | 329421 | Myo3b | myosin IIIB | XM_017319045.1 | 56% | 72.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 98
- ORF end:
- 3428
- ORF length:
- 3330
- Sequence:
-
1 AAAGATTTTA TTTTGACTGA TAGTGACCTG TTCGTTGCAA CAAATTGATG AGCAATGCTT 61 TTTTATAATG CCAACTTTGT ACAAAAAAGT TGGCACCATG AAACATCTGT ATGGATTATT 121 TCACTATAAT CCTATGATGC TTGGACTTGA ATCACTTCCA GATCCCACAG ACACCTGGGA 181 AATTATAGAG ACCATTGGTA AAGGCACCTA TGGCAAAGTC TACAAGGTAA CTAACAAGAG 241 AGATGGGAGC CTGGCTGCAG TGAAAATTCT GGATCCAGTC AGTGATATGG ATGAAGAAAT 301 TGAGGCAGAA TACAACATTT TGCAGTTCCT TCCTAATCAT CCCAATGTTG TAAAGTTTTA 361 TGGGATGTTT TACAAAGCGG ATCACTGTGT AGGGGGACAG CTGTGGCTGG TCCTGGAGCT 421 GTGTAATGGG GGCTCAGTCA CTGAGCTTGT CAAAGGTCTA CTCAGATGTG GCCAGCGGTT 481 GGATGAAGCA ATGATCTCAT ACATCTTGTA CGGGGCCCTC TTGGGCCTTC AGCATTTGCA 541 CAACAACCGA ATCATCCACC GTGATGTGAA GGGGAATAAC ATTCTTCTGA CAACAGAAGG 601 AGGAGTTAAG CTCGTTGACT TTGGTGTTTC AGCTCAACTC ACCAGTACAC GTCTGCGGAG 661 AAACACATCT GTTGGCACCC CGTTCTGGAT GGCCCCTGAG GTCATTGCCT GTGAGCAGCA 721 GTATGACTCT TCCTATGACG CTCGCTGTGA CGTCTGGTCC TTGGGGATCA CAGCTATTGA 781 ACTGGGGGAT GGAGACCCTC CCCTCTTTGA CATGCATCCT GTGaaaacac tctttaagat 841 tccaagaaat cctccaccta ctttacttca tccagaaaaa tggtgtgaag aattcaacca 901 ctttatttca cagtgtctta ttaaggattt tgaaaggcga ccttccgtca cacatctcct 961 tgaccaccca tttattaaag gagtacatgg aaaagttctg tttctgcaaa aacagctggc 1021 caaggttctc caagaccaga agcatcaaaa tcctgttgct aaaaccaggc atgagaggat 1081 gcataccaga agaccttatc atgtggaaga tgctgaaaaa tactgccttg aggatgattt 1141 ggtcaaccta gaggttctgg atgaggatac aattatccat cagttgcaga aacgttatgc 1201 agacttgcta atttacacat atgttggaga catcttaatt gccttaaacc ccttccagaa 1261 tctaagcata tactctccac agttttccag actttatcat ggggtgaaac gcgcctccaa 1321 tcccccccac atatttgcat cagcagatgc tgcttaccag tgcatggtta ctctcagcaa 1381 agaccagtgc attgtcatca gcggagagag tggctctggg aagacagaaa gcgcccacct 1441 gattgttcaa catttgactt tcttgggaaa ggccaataat cagaccttga gagagaaaat 1501 tctacaagtc aactccctgg tggaagcctt tgggaactca tgcactgcca tcaatgacaa 1561 ctcgagccgt tttggaaaat atctggaaat gatgtttaca ccaactggag ttgtgatggg 1621 ggcaagaatc tctgaatatc tcctggaaaa atccagagtt ataaaacagg cagcgagaga 1681 gaaaaatttt catatatttt actatattta tgctggtctt catcaccaaa agaagctttc 1741 tgatttcaga cttcctgagg aaaaacctcc taggtacata gctgatgaaa ctggaagggt 1801 gatgcacgac ataacttcca aggagtctta cagaagacaa ttcgaagcaa ttcagcattg 1861 cttcaggatt atagggttca cggacaaaga ggtgcactca gtgtacagaa ttttggctgg 1921 gattttgaat attgggaaca ttgagttcgc agctatttcc tctcaacatc agactgataa 1981 aagtgaggtg cccaatgctg aagctttgca aaatgctgcc tctgttctgt gcattagccc 2041 tgaagagctc caggaggccc tcacctccca ctgtgtggtc acccggggcg agaccatcat 2101 ccgtgccaac actgtagaca gggctgcgga cgttcgagac gccatgtcca aagccctgta 2161 tgggaggctc ttcagctgga ttgtgaatcg cattaataca ctcctgcagc cagacgaaaa 2221 catatgtagt gcaggaggtg gaatgaatgt ggggatcttg gatatctttg gattcgagaa 2281 ttttcagaga aattcatttg agcagctctg cataaacatc gccaatgagc aaatccagta 2341 ctatttcaat cagcatgttt ttgctcttga gcagatggaa tatcagaatg aaggcattga 2401 tgctgtaccc gtggaatatg aggacaaccg cccgctcttg gacatgttcc tccagaaacc 2461 cctgggactg cttgcacttt tggatgagga aagtcggttt ccccaagcaa ctgaccagac 2521 cctggttgat aaatttgaag ataatctacg atgcaaatac ttctggaggc ccaaaggagt 2581 ggaactgtgc tttggcattc agcattatgc tggaaaggta ttatatgatg cttctggggt 2641 tcttgagaaa aatagagaca ctctccctgc cgatgtggtt gtggtcctga gaacgtcaga 2701 aaacatgctt cttcagcagc tcttctcaat ccctctgacc aaaacaggta atttggccca 2761 gacaagagct aggataacag tggcctcaag ttctttgcct ccacatttca gtgctgggaa 2821 agccaaggtg gacactctgg aggtgatacg gcatccggaa gaaaccacca acatgaagag 2881 gcaaactgtg gcttcttact tccggtattc tctgatggac ctgctctcca aaatggtggt 2941 tggacagccc cactttgtgc gctgcattaa acccaatgat gaccgagagg ccctgcagtt 3001 ctctcgagag agggtgctgg cccagctccg ctccacaggg attctggaga cagtcagcat 3061 ccgccgccag ggctattccc accgcatcct ttttgaagaa tttgtgaaaa ggtattatta 3121 cttggcattc acagcacatc aaacacctct tgctagcaaa gagagctgtg tggctatctt 3181 ggaaaagtcc agattagatc actgggtgct gggaaaaaca aaggtttttc tcaaatatta 3241 ccatgttgag caattaaatt tgctgcttcg agaagtcata ggcagagtgg ttgtgctgca 3301 ggcatatacc aaggggtggc ttggagccag gagatacaaa aaggtcagag agaagagaga 3361 gaagggagcc attgccATCC CAGTCAGCCT TGGAGAGGAT ATGATGCTCG GAGGAAATTT 3421 AAGAAAATAA GCAACAGAAG GAATGAGTCT GCTGCTCATA ATCAAGCAGG GGACACTTCA 3481 AACCAAAGCA GTGGGCCACA TTCCCCCGTC GCAGCAGGTA CGAGGGGAAG TGCCGAGGTT 3541 CAAGACTGCA GCGAGCCTGG TGACCATAAA GTTCTCAGGG GCTCTGTACA TCGTAGGAGC 3601 CATTCACAAG CAGAATCCAA CAATGGCCGT ACACAGACTT CAAGCAACTC TCCTGCTGTC 3661 ACAGAGAAAA ATGGGCATTC ACAAGCCCAG AGTTCTCCAA AAGGGTGCGA TATCTTCGCA 3721 GGACATGCAA ACAAGCACTC GGTTTCTGGG ACTGATTTGC TGTCTTCTCG GATATGCCAT 3781 CCTGCTCCAG ATCAGCAAGG ATTGAGTCTC TGGGGAGCCC CTCAAAAGCC TGGTTCAGAA 3841 AATGGTCTTG CACAGAAGCA TCGAACACCT CGCCGACGAT GTCAGCAGCC CAAAATGCTG 3901 AGTAGCCCTG AGGACACCAT GTACTATAAC CAGTTAAATG GAACTCTAGA ATATCAAGGG 3961 AGCAAGAGGA AGCCAAGAAA ACTTGGCCAA ATCAAAGTAC TTGATGGGGA AGATGAATAT 4021 TACAAATCTC TGTCACCAGT GGACTGTATC CCTGAGGAGA ACAACTCAGC CCACCCTTCC 4081 TTTTTTTCTT CATCCTCAAA AGGAGACTCT TTTGCTCAAC ATTTGCCAAC TTTCTTGTAC 4141 AAAGTTGGCA TTATAAGAAA GCATTGCTTA TCAATTTGTT GCAACGAACA GGTCACTATC 4201 AGTCAAAATA AAATCATTAT TGCCATCCAG CGATCATCGG T