Construct: ORF ccsbBroadEn_15305
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018834.1_s300c1, BRDN0000458130
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- MYLK4 (340156)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 340156 | MYLK4 | myosin light chain kinase f... | NM_001012418.5 | 98.4% | 30.9% | (many diffs) |
| 2 | human | 340156 | MYLK4 | myosin light chain kinase f... | XM_005249079.2 | 98.4% | 30.9% | (many diffs) |
| 3 | human | 340156 | MYLK4 | myosin light chain kinase f... | XM_006715082.3 | 89% | 19.1% | (many diffs) |
| 4 | human | 340156 | MYLK4 | myosin light chain kinase f... | NM_001347872.2 | 86.1% | 27% | (many diffs) |
| 5 | human | 340156 | MYLK4 | myosin light chain kinase f... | XM_005249078.4 | 81.5% | 25.5% | (many diffs) |
| 6 | human | 340156 | MYLK4 | myosin light chain kinase f... | XR_926185.3 | 36.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 98
- ORF end:
- 488
- ORF length:
- 390
- Sequence:
-
1 TTGGATTTTA TTTTGACTGA TAGTGACCTG TTCGTTGCAA CAAATTGATG AGCAATGCTT 61 TTTTATAATG CCAACTTTGT ACAAAAAAGT TGGCACCATG TTAAAAGTGA AGAGGCTGGA 121 AGAATTCAAC ACGTGTTATA ACAGCAACCA GCTGGAGAAA ATGGCCTTTT TTCAGTGCAG 181 GGAAGAGGTG GAGAAAGTGA AGTGTTTTCT GGAAAAAAAT TCTGGGGACC AGGATTCAAG 241 ATCTGGACAT AATGAGGCGA AGGAGGTGTG GTCAAACGCC GACCTGACGG AAAGGATGCC 301 CGTCAAAAGC AAAAGGACAT CAGCCCTCGC AGTTGACATC CCGGCTCCTC CGGCCCCATT 361 TGATCATCGT ATTGTGACAG CCAAGCAAGG AGCGGTCAAC AGCTTCTATA CTGTGAGCAA 421 GACAGAAATC CTAGGAGGAG GGCGTTTCGG CCAGNTTCAC CAAGTGGNGA GGAGACGGCC 481 ACAGGTCTGA AGCTGGCAGC CAAAATCATC AAGACCAGAG GCATGAAGNN NNAGNNNGAG 541 GTGAAGAACG AGATCAGCGT CATGAACCAG CTGGACCACG CGAACCTCAT CCAGCTGTAC 601 GATGCNTTCG AGTCTAAGAA CGACATTGTC CTGGTCATGG AGTATGTGGA TGGTGGGGGA 661 GCTGTTTGAC CGCATCATCG ATGAGAGCTA CAATTTGACG GAGCTTGATA CCATCCTGTT 721 CATGAAGCAG ATATGTGAGG GGATAAGGCA CATGCATCAG ATGTACATTC TCCACTTGGA 781 CCTGAAGCCT GAGAATATCC TGTGTGTGAA TCGGGATGCT AAGCAAATAA AAATTATTGA 841 TTTTGGGATT GGCCAGAAGA TACAAACCCA GAGAGAAGCT GAAGGTGAAC TTTGGAACCC 901 CAGAATTTCT CCGNCCCCTG AAGTTGTGAA CTTATGATTT TGTTTCATTT CCCACTGACA 961 TGTGGAGTGT GGGGGTCATC GCCTATATGC TACTTAGCGG TTTGTCGCCT TTCCTGGGTG 1021 ACAATGATGC TGAGACGCTG AACAACATCC TGGCCTGCAG GTGGGACTTA GAGGATGAAG 1081 AATTTCAGGA CATCTCGGAG GAGGCCAAGG AGTTCATCTC TAAGCTTCTG ATTAAGGAGA 1141 AGAGTTGGCG AATAAGTGCA AGCGAAGCTC TCAAGCACCC CTGGTTGTCA GACCACAAGC 1201 TCCACTCCAG ACTCAATGCC CAGAAGAAGA AGAATCGTGG CTCTGATGCC CAGGACTTTG 1261 TGACCAAATT GCCAACTTTC TTGTACAAAG TTGGCATTAT AAGAAAGCAT TGCTTATCAA 1321 TTTGTTGCAA CGAACAGGTC ACTATCAGTC AAAATAAAAT CATTATTTGC CATCCAGCGA 1381 TGCCCA