Construct: ORF ccsbBroadEn_15322
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018935.1_s300c1, BRDN0000458231
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- MAP3K15 (389840)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 389840 | MAP3K15 | mitogen-activated protein k... | XM_011545511.2 | 98.9% | 1.2% | 0_1ins22;1598delC |
| 2 | human | 389840 | MAP3K15 | mitogen-activated protein k... | XM_011545510.2 | 86.6% | 1% | 1_347del;1967delC |
| 3 | human | 389840 | MAP3K15 | mitogen-activated protein k... | XM_011545507.3 | 63% | .7% | 1_1328del;2948delC |
| 4 | human | 389840 | MAP3K15 | mitogen-activated protein k... | XM_011545508.3 | 60.6% | .7% | 1_1328del;2425_2426ins87;2861delC |
| 5 | human | 389840 | MAP3K15 | mitogen-activated protein k... | NM_001001671.3 | 57.5% | .6% | 1_1673del;3293delC |
| 6 | mouse | 270672 | Map3k15 | mitogen-activated protein k... | NM_001163085.2 | 48.3% | .5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 82
- ORF end:
- 169
- ORF length:
- 87
- Sequence:
-
1 CTGATAGTGA CCTGTTCGTT GCAACAAATT GATGAGCAAT GCTTTTTTAT AATGCCAACT 61 TTGTACAAAA AAGTTGGCAC CATGGCATGT CTCACCCACA GAAATGAAAC AGATGCACGA 121 ATGGAATTTT ACAGCCTCTT CCATAAAGGG AATAAGCCTA TCAAAGTTTG ATGAAAGGTG 181 TTGTTTTCTT TATGTCCATG ATAATTCTGA TGACTTTCAA ATCTACTTTT CCACCGAAGA 241 GCAGTGCAGT AGATTTTTCT CTTTGGTCAA AGAGATGATA ACCAATACAG CAGGCAGTAC 301 GGTGGAGCTG GAGGGAGAGA CCGATGGAGA CACCTTGGAG TATGAGTATG ACCATGATGC 361 AAATGGTGAG AGAGTTGTCT TGGGGAAAGG CACGTATGGG ATTGTGTATG CTGGCCGAGA 421 TCTGAGCAAT CAAGTGCGAA TAGCCATCAA AGAAATCCCG GAGAGAGATA GCAGGTATTC 481 TCAGCCTCTG CACGAGGAGA TAGCCCTGCA CAAGTACCTT AAGCACCGCA ATATCGTTCA 541 GTACCTGGGC TCTGTTTCAG AGAACGGCTA CATTAAGATA TTTATGGAGC AGGTGCCTGG 601 AGGAAGCCTT TCTGCTCTTC TGCGATCCAA ATGGGGGCCG ATGAAGGAAC CGACAATCAA 661 GTTTTACACC AAACAGATCC TGGAGGGCCT TAAGTATCTT CATGAAAACC AGATCGTGCA 721 CAGAGACATA AAGGGCGATA ATGTTCTGGT GAACACCTAC AGCGGAGTGG TGaaaatctc 781 cgattttgga acctcgaaac gtcttgcggg tgtgaacccc tgcacagaga cttttactgg 841 caccctgcag tacatggcac ctgagataat tgaccaaggg cctcgcggat atggtgcccc 901 agccgatatc tggtccctgg gctgcaccat cattgagatg gccaccagca agcctccgtt 961 ccatgagctt ggtgagccgc aggcagccat gttcaaagtg ggcatgttta agatccaccc 1021 tgagattcca gaagcccttt cagctgaagc ccgagccttc attttatcct gtttcgagcc 1081 tgacccccac aaacgtgcca ccactgctga gctactgaga gagggtttct taaggcaggt 1141 gaacaagggc aagaagaacc gaattgcctt caagccctca gaaggtcccc gcggtgtcgt 1201 cctggccctg cccacacagg gagagcccat ggccaccagc agcagcgagc acggctctgt 1261 ctccccagac tccgacgccc agcctgacgc actctttgag aggacccggg cgcccaggca 1321 ccaccttggc cacctcctca gtgttccaga cgagagctca gccttggaag accggggctt 1381 ggcctcgtcc ccggaggaca gggaccaggg cctcttcctg ctacgcaagg acagtgagcg 1441 ccgtgccatc ctgtacaaaa tcctctggga ggagcagaac caggtggctt ccaacctgca 1501 ggagtgtgtg gcccagagtt ccgaagagtt gcatctctca gttggacaca tcaagcaaat 1561 cattgggatc ctgagggact tcatccgctc cccagagcac cgggtgatgg cgaccacaat 1621 atcaaagcTC AAGGTGGACC TGGACTTTGA CAGCTCGTCC ATCAGTCAGA TTCACCTGGT 1681 GCTGTTCGGA TTTCAGGATG CGTAAATAAA ATTTTGAGGA ACCACTTAAT TAGGCCCCAC 1741 TGGATGTTCG CGATGGACAA CATCATCCGC CGAGCGGTGC AGGCCGCGGT CACCATTCTC 1801 ATCCCAGAGC TCCGAGCCCA CTTTGAGCCT ACCTGTGAGA CTGAAGGGGT AGATAAGGAC 1861 ATGGATGAAG CGGAAGAGGG CTATCCCCCA GCCACCGGAC CTGGCCAGGA GGCCCAGCCC 1921 CACCAGCAGC ACCTGAGCCT CCAGCTGGGT GAGCTCAGAC AGGAGACCAA CAGACTTTTG 1981 GAACACCTAG TTGAAAAAGA GAGAGAGTAC CAGAATCTTC TGCGGCAAAC TCTAGAACAG 2041 AAAACTCAAG AATTGTATCA CCTTCAGTTA AAATTAAAAT CGAATTGTAT TACAGAGAAC 2101 CCAGCAGGCC CCTACGGGCA GAGAACAGAT AAAGAGCTTA TAGACTGGTT GCGGCTGCAA 2161 GGAGCTGATG CAAAGACAAT TGAAAAGATT GTTGAAGAGG GTTATACACT TTCGGATATT 2221 CTTAATGAGA TCACTAAGGA AGATCTAAGA TACCTTCGAC TACGGGGTGG TCTCCTCTGC 2281 AGACTCTGGA GTGCGGTCTC CCAGTACAGA AGGGCTCAGG AGGCCTCAGA AACCAAAGAC 2341 AAGGCTTTGC CAACTTTCTT GTACAAAGTT GGCATTATAA GAAAGCATTG CTTATCAATT 2401 TGTTGCAACG AACAGGTCAC TATCAGTCAA AATAAAATCA TTATTGCCAT CCAGCCTTTC 2461 CCCG