Construct: ORF ccsbBroadEn_15334
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018949.1_s300c1, BRDN0000458245
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LOC648152 (648152)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 545 | ATR | ATR serine/threonine kinase | NM_001354579.2 | 14.6% | 14.5% | (many diffs) |
| 2 | human | 545 | ATR | ATR serine/threonine kinase | XM_011512925.1 | 14.6% | 14.5% | (many diffs) |
| 3 | human | 545 | ATR | ATR serine/threonine kinase | NM_001184.4 | 14.2% | 14.2% | (many diffs) |
| 4 | human | 545 | ATR | ATR serine/threonine kinase | XM_011512924.1 | 14.2% | 14.2% | (many diffs) |
| 5 | human | 545 | ATR | ATR serine/threonine kinase | XR_001740179.2 | 13.6% | (many diffs) | |
| 6 | human | 545 | ATR | ATR serine/threonine kinase | XR_924148.2 | 13.6% | (many diffs) | |
| 7 | mouse | 245000 | Atr | ataxia telangiectasia and R... | XM_006511188.3 | 25.5% | 26.6% | (many diffs) |
| 8 | mouse | 245000 | Atr | ataxia telangiectasia and R... | XM_006511184.3 | 13% | 13.6% | (many diffs) |
| 9 | mouse | 245000 | Atr | ataxia telangiectasia and R... | NM_019864.1 | 13% | 13.6% | (many diffs) |
| 10 | mouse | 245000 | Atr | ataxia telangiectasia and R... | XR_001778905.1 | 11.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 82
- ORF end:
- 1219
- ORF length:
- 1137
- Sequence:
-
1 CGGATAGTGA CCTGTTCGTT GCAACAAATT GATGAGCAAT GCTTTTTTAT AATGCCAACT 61 TTGTACAAAA AAGTTGGCAC CATGATACCT ACACTTCCAT CAATTCTGGG TACCCATGCT 121 AACCATGCTA GCCATGAACC ATTTCCTGGA CATTGGGCCT ATATTGCAGG GTTTGATGAT 181 ATGGTGGAAA TTCTTGCTTC TCTTCAGAAA CCAAAGAAGA TTTCTTTAAA AGGCTCAGAT 241 GGAAAGTTCT ACATCATGAT GTGTAAGCCA AAAGATGACC TGAGAAAGGA TTGTAGACTA 301 ATGGAATTCA ATTCCTTGAT TAATAAGTGC TTAAGAAAAG ATGCAGAGTC TCGTAGAAGA 361 GAACTTCATA TTCGAACATA TGCAGTTATT CCACTAAATG ATGAATGTGG GATTATTGAA 421 TGGGTGAACN ACACTGCTGG TTTGAGACCT ATTCTGACCA AACTATATAA AGAAAAGGGA 481 GTGTATATGA CAGGAAAANN ACTTCGCCAG TGTATGCTAC CAAAGTCAGC AGCTTTATCT 541 GAAAAACTCA AAGTATTCCG AGAATTTCTC CTGCCCAGGC ATCCTCCTAT TTTTCATGAG 601 TGGTTTCTGA GAACATTCCC TGATCNTACA TCATGGTACA GTAGTAGATC AGCTTACTGC 661 CGTTCCACTG CAGTAATGTC AATGGTTGGT TATATTCTGG GGCTTGGAGA CCGTCATGGT 721 GAAAATATTC TCTTTGATTC TTTGACTGGT GAATGCGTAC ATGTAGATTT CAATTGTCTT 781 TTCAATAAGG GAGAAACCTT TGAAGTTCCA GAAATTGTGC CATTTCGCCT GACTCATAAT 841 ATGGTTAATG GAATGGGTCC TATGGGAACA GAGGGTCTTT TTCGAAGAGC ATGTGAAGTT 901 ACAATGAGGC TGATGCGTGA TCAGCGAGAG CCTTTAATGA GTGTCTTAAA GACTTTTCTA 961 CATGATCCTC TTGTGGAATG GAGTAAACCA GTGAAAGGGC ATTCCAAAGC GCCACTGAAT 1021 GAAACTGGAG AAGTTGTCAA TGAAAAGGCC AAGACCCATG TTCTTGACAT TGAGCAGCGA 1081 CTACAAGGTG TAATCAAGAC TCGAAATAGA GTGACAGGAC TGCCGTTATC TATTGAAGGA 1141 CATGTGCATT ACCTTATACA AGAAGCTACT GATGAAAACT TACTATGCCA GATGTATCTT 1201 GGTTGGACTC CATATATGTT GCCAACTTTC TTGTACAAAG TTGGCATTAT AAGAAAGCAT 1261 TGCTTATCAA TTTGTTGCAA CGAACAGGTC ACTATCAGTC AAAATAAAAT CATTATTGCC 1321 ATCCAGCTAG CTGAGG