Construct: ORF ccsbBroadEn_15336
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018802.1_s300c1, BRDN0000458098
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LOC650122 (650122)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1119 | CHKA | choline kinase alpha | XM_017017148.2 | 86.9% | 84.6% | (many diffs) |
| 2 | human | 1119 | CHKA | choline kinase alpha | NM_212469.1 | 71% | 69.4% | (many diffs) |
| 3 | human | 1119 | CHKA | choline kinase alpha | NM_001277.3 | 68.3% | 66.7% | (many diffs) |
| 4 | human | 1119 | CHKA | choline kinase alpha | XM_017017147.1 | 64.9% | 60.9% | (many diffs) |
| 5 | human | 1119 | CHKA | choline kinase alpha | XR_428904.3 | 47.2% | (many diffs) | |
| 6 | human | 1119 | CHKA | choline kinase alpha | XR_949772.2 | 32.6% | (many diffs) | |
| 7 | human | 1119 | CHKA | choline kinase alpha | XR_949773.2 | 29.7% | (many diffs) | |
| 8 | human | 1119 | CHKA | choline kinase alpha | XR_428905.3 | 10.8% | (many diffs) | |
| 9 | mouse | 12660 | Chka | choline kinase alpha | XM_006531648.2 | 75.2% | 74.6% | (many diffs) |
| 10 | mouse | 12660 | Chka | choline kinase alpha | XM_006531649.2 | 75.2% | 74.6% | (many diffs) |
| 11 | mouse | 12660 | Chka | choline kinase alpha | XM_006531650.2 | 75.2% | 74.6% | (many diffs) |
| 12 | mouse | 12660 | Chka | choline kinase alpha | XM_006531651.3 | 75.2% | 74.6% | (many diffs) |
| 13 | mouse | 12660 | Chka | choline kinase alpha | XM_011248599.2 | 75.2% | 74.6% | (many diffs) |
| 14 | mouse | 12660 | Chka | choline kinase alpha | XM_011248600.2 | 75.2% | 74.6% | (many diffs) |
| 15 | mouse | 12660 | Chka | choline kinase alpha | XM_017318048.1 | 75.2% | 74.6% | (many diffs) |
| 16 | mouse | 12660 | Chka | choline kinase alpha | XM_017318049.1 | 75.2% | 74.6% | (many diffs) |
| 17 | mouse | 12660 | Chka | choline kinase alpha | XM_017318050.1 | 75.2% | 74.6% | (many diffs) |
| 18 | mouse | 12660 | Chka | choline kinase alpha | NM_013490.4 | 62% | 61.6% | (many diffs) |
| 19 | mouse | 12660 | Chka | choline kinase alpha | NM_001271496.1 | 59.6% | 59.2% | (many diffs) |
| 20 | mouse | 12660 | Chka | choline kinase alpha | XM_006531646.1 | 58.3% | 57.9% | (many diffs) |
| 21 | mouse | 12660 | Chka | choline kinase alpha | XR_388248.2 | 35.4% | (many diffs) | |
| 22 | mouse | 12660 | Chka | choline kinase alpha | NR_073190.1 | 30.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 96
- ORF end:
- 1056
- ORF length:
- 960
- Sequence:
-
1 GGTTTTATTT TGACTGGATA GTGACCTGTT CGTTGCAACA AATTGATGAG CAATGCTTTT 61 TTATAATGCC AACTTTGTAC AAAAAAGTTG GCACCATGCT GTTCCAGTGC TCCCTACCTG 121 ACACCACAGC CACCCTTGGT GATGAGCCTC GGAAAGTGCT CCTGCGGCTG TATGGAGCGA 181 TTTTGCAGAT GGGGGCTGAG GCCATGGTTC TGGAGAGCGT TATGTTTGCC ATTCTCGCAG 241 AGAGGTCACT TGGGCCAAAA CTCTATGGCA TCTTTCCCCA AGGCCGACTG GAGCAGTTCA 301 TCCCGAGCCG GCGATTAGAT ACTGAAGAAT TAAGTTTGCC AGATATTTCT GCAGAAATCG 361 CCGAGAAAAT GGCTACATTT CATGGTATGA AAATGCCATT CAATAAGGAA CCAAAATGGC 421 TTTTTGGCAC AATGGAAAAG TATCTAAAGG AAGTGCTGAG AATTAAATTT ACTGAGGAAT 481 CCAGAATTAA AAAGCTCCAC AAATTGCTCA GTTACAATCT GCCCTTGGAA CTGGAAAACC 541 TGAGATCATT GCTTGAATCT ACTCCATCTC CAGTTGTATT TTGTCATAAT GACTGTCAAG 601 AAGGTAATAT CTTGTTGCTG GAAGGCCGAG AGAATTCTGA AAAACAGAAA CTGATGCTCA 661 TTGATTTCGA ATACAGCAGT TACAATTACA GGGGATTCGA CATTGGAAAT CACTTCTGTG 721 AGTGGATGTA TGATTATAGC TATGAAAAAT ACCCTTTTTT CAGAGCAANN NNCCGGAAGT 781 ATCCCACCAA GAAACAACAG CTCCATTTTA TTTCCAGTTA CTTGCCTGCA TTCCAAAATG 841 ACTTTGAAAA CCTCAGTACT GAAGAAAAAT CCATTATAAA AGAAGAAATG TTGCTTGAAG 901 TTAATAGGTT TGCCCTTGCA TCTCATTTCC TCTGGGGACT GTGGTCCATT GTACAAGCCA 961 AGATTTCATC TATTGAATTT GGGTACATGN NCNACGCCCA AGCAAGGTTT GATGCCTATT 1021 TCCAGTTACT TGCCNGNAGA GGAAGCTTGG GGTGTTNCNA ANTNNCTTNN ANAANNTNNG 1081 TACTGAAGAA AAATCCATTA TAAGAAAGNA GAAATGTTGC TTNTCAATTT GTTGCAACGA 1141 ACAGGTCACT ATCAGTCAAA ATAAAATCAT TATTGCCATC CAGCTGATTC CCA