Construct: ORF ccsbBroadEn_15340

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018793.1_s300c1, BRDN0000458089
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
LOC653155 (653155)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 8899 PRPF4B pre-mRNA processing factor 4B NM_003913.5 10.4% 10.5% 1_2703del;3000C>T
2 human 8899 PRPF4B pre-mRNA processing factor 4B XM_017011410.2 10.4% 10.5% 1_2703del;3000C>T
3 human 8899 PRPF4B pre-mRNA processing factor 4B XM_017011411.2 10.4% 10.5% 1_2703del;3000C>T
4 human 8899 PRPF4B pre-mRNA processing factor 4B XM_017011413.2 10.4% 10.5% 1_2703del;3000C>T
5 human 8899 PRPF4B pre-mRNA processing factor 4B XM_017011414.2 10.4% 10.5% 1_2703del;3000C>T
6 human 8899 PRPF4B pre-mRNA processing factor 4B XM_017011415.2 10.4% 10.5% 1_2703del;3000C>T
7 human 8899 PRPF4B pre-mRNA processing factor 4B XM_017011416.2 10.4% 10.5% 1_2703del;3000C>T
8 human 8899 PRPF4B pre-mRNA processing factor 4B XM_017011417.2 10.4% 10.5% 1_2703del;3000C>T
9 human 8899 PRPF4B pre-mRNA processing factor 4B XM_017011418.2 10.4% 10.5% 1_2703del;3000C>T
10 human 8899 PRPF4B pre-mRNA processing factor 4B XM_017011420.2 10.4% 10.5% 1_2703del;3000C>T
11 human 8899 PRPF4B pre-mRNA processing factor 4B XM_017011421.2 10.4% 10.5% 1_2703del;3000C>T
12 human 8899 PRPF4B pre-mRNA processing factor 4B XM_017011422.2 10.4% 10.5% 1_2703del;3000C>T
13 human 8899 PRPF4B pre-mRNA processing factor 4B XM_017011423.2 10.4% 10.5% 1_2703del;3000C>T
14 human 8899 PRPF4B pre-mRNA processing factor 4B XM_017011424.2 10.4% 10.5% 1_2703del;3000C>T
15 human 8899 PRPF4B pre-mRNA processing factor 4B XM_017011425.2 10.4% 10.5% 1_2703del;3000C>T
16 human 8899 PRPF4B pre-mRNA processing factor 4B XM_017011426.2 10.4% 10.5% 1_2703del;3000C>T
17 human 8899 PRPF4B pre-mRNA processing factor 4B XM_017011427.2 10.4% 10.5% 1_2703del;3000C>T
18 human 8899 PRPF4B pre-mRNA processing factor 4B XM_017011428.2 10.4% 10.5% 1_2703del;3000C>T
19 human 8899 PRPF4B pre-mRNA processing factor 4B XM_017011430.2 10.4% 10.5% 1_2703del;3000C>T
20 human 8899 PRPF4B pre-mRNA processing factor 4B NR_146784.2 8.8% 1_2829del;3126C>T;3148_3582del
21 human 8899 PRPF4B pre-mRNA processing factor 4B NR_146783.2 8.5% 1_2829del;3126C>T;3148_3715del
22 human 8899 PRPF4B pre-mRNA processing factor 4B NR_146785.2 8.3% 1_2829del;3126C>T;3148_3792del
23 mouse 19134 Prpf4b pre-mRNA processing factor 4B NM_013830.2 9.8% 10.5% (many diffs)
24 mouse 19134 Prpf4b pre-mRNA processing factor 4B XM_017315425.1 9.8% 10.5% (many diffs)
25 mouse 19134 Prpf4b pre-mRNA processing factor 4B XM_017315426.1 9.8% 10.5% (many diffs)
26 mouse 19134 Prpf4b pre-mRNA processing factor 4B XM_017315427.1 9.8% 10.5% (many diffs)
27 mouse 19134 Prpf4b pre-mRNA processing factor 4B XM_017315428.1 9.8% 10.5% (many diffs)
28 mouse 19134 Prpf4b pre-mRNA processing factor 4B XM_017315429.1 9.8% 10.5% (many diffs)
29 mouse 19134 Prpf4b pre-mRNA processing factor 4B XM_017315430.1 9.8% 10.5% (many diffs)
30 mouse 19134 Prpf4b pre-mRNA processing factor 4B XM_017315431.1 9.8% 10.5% (many diffs)
31 mouse 19134 Prpf4b pre-mRNA processing factor 4B XM_017315432.1 9.8% 10.5% (many diffs)
32 mouse 19134 Prpf4b pre-mRNA processing factor 4B XM_017315433.1 9.8% 10.5% (many diffs)
33 mouse 19134 Prpf4b pre-mRNA processing factor 4B XM_017315434.1 9.8% 10.5% (many diffs)
34 mouse 19134 Prpf4b pre-mRNA processing factor 4B XM_017315435.1 9.8% 10.5% (many diffs)
35 mouse 19134 Prpf4b pre-mRNA processing factor 4B XM_017315436.1 9.8% 10.5% (many diffs)
36 mouse 19134 Prpf4b pre-mRNA processing factor 4B XM_017315437.1 9.8% 10.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
466
ORF end:
784
ORF length:
318
Sequence:
1NNNNNNTNNN NTNNGNNNGN NNGTGNNNNT GATGGGTGGG ATTGCGGAGA CGCTGGCACC
61ACCTATAAAA AAAAAAAAAA GTAAGACTAG CATATTTCAC AGAGACGTGG ATGACGTCGA
121TCGAGAGATC AAGACATTTG ACAGGTTTGA TTTATTGGAA GCTAAGCTTA TTAGTGAACC
181ACATGAAGTA ATTTAGTCTA ACGATTAAAC AAGTCACACC TCAACGAATT AATTTTTGTT
241TTCTTAAGTA CATGAACAAT GTTTAATGCC CTCTCATTTT TTTTTTTTTT TATTGTCTAC
301TTCTTTTTAT TTTTCCCAGT CACAGATGTT GTAAACACGA CGGCCAGTAT TAAGGCTGGG
361CCCCAAATAA TGATTTTATT TTGACTGATA GTGACCTGTT CGTTGCAACA AATTGATGAG
421CAATGCTTTT TTATAATGCC AACTTTGTAC AAAAAAGTTG GCACCATGGA TCTCAAAGGA
481AAGATGCCAA ATAAGATGAT TCGAAAAGGT GTGTTCAAAG ATCAGCATTT TGATCAAAAT
541CTCAACTTCA TGTACATAGA AGTTGATAAA GTAACAGAGA GGGAGAAAGT TACTGTTATG
601AGCACCATTA ATCCAACTAA GGACCTGTTG GCTGACTTGA TTGGGTGCCA GAGACTTCCT
661GAAGACCAAC GTAAGAAAGT ACACCAGCTA AAGGACTTGT TGGACCAGAT TCTGATGTTG
721GACCCAGCTA AACGAATTAG CATCAACCAG GCCCTACAGC ATGCCTTCAT CCAGGAAAAA
781ATTTTGCCAA CTTTCTTGTA CAAAGTTGGC ATTATAAGAA AGCATTGCTT ATCAATTTGT
841TGCAACGAAC AGGTCACTAT CAGTCAAAAT AAAATCATTA TTNNNNNNCN NNNNNNTNNN
901NCCTATAGTG AGTCGTATTA CATGGTCATA GCTGTTTCCT GGCAGCTCTG GCCCGTGTCT
961CAAAATCTCA GAATGTTACA CTGCCCAGAA ATGTACCGGG CTCAGTAATA TAACCCTGAA
1021GTCCCTACAG AAAATAAGCT GTCTCTGAGC ACGCACAAAA GACGATCAAG AATCCGCCCC
1081GCATTCTACC CCCTATTCTA TGACAGGCTC AACGGAACAG GCCCAAAAGC ATATCATCCC
1141TCCCTTCTTT GCGTATGGAG CGAATTCGCT AGAGCTCAGT ATCTTTGGTG ATAGGTACGG
1201GTGCTGTCGA GCAATCCAGC CTACCGTGCC TACTGCTTAA CTCCTGCCTT AGTAAATATT
1261ATATATTTGT GCAATTTTTT CGACAA

Download FASTA (ORF) (Full)