Construct: ORF ccsbBroadEn_15367

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006579.1_s300c1, BRDN0000387780
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
BARD1 (580)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 580 BARD1 BRCA1 associated RING domain 1 NM_000465.4 99.7% 99.6% (many diffs)
2 human 580 BARD1 BRCA1 associated RING domain 1 NM_001282543.2 97.3% 97.1% (many diffs)
3 human 580 BARD1 BRCA1 associated RING domain 1 XM_017004613.1 95.7% 95.4% (many diffs)
4 human 580 BARD1 BRCA1 associated RING domain 1 XR_002959322.1 81.9% (many diffs)
5 human 580 BARD1 BRCA1 associated RING domain 1 XM_017004614.1 78% 77.9% (many diffs)
6 human 580 BARD1 BRCA1 associated RING domain 1 NM_001282545.2 41.8% 41.8% 70C>T;84G>T;215_216ins1353
7 human 580 BARD1 BRCA1 associated RING domain 1 NR_104212.2 39.7% (many diffs)
8 human 580 BARD1 BRCA1 associated RING domain 1 NR_104215.2 38.7% (many diffs)
9 human 580 BARD1 BRCA1 associated RING domain 1 NM_001282548.2 36% 39.3% (many diffs)
10 human 580 BARD1 BRCA1 associated RING domain 1 NM_001282549.2 33.8% 33.7% 70C>T;84G>T;364_365ins1539
11 human 580 BARD1 BRCA1 associated RING domain 1 NR_104216.2 25.1% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2400
ORF length:
2331
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag
61ttggcaccat gccggataat cggcagccga ggaaccggca gccgaggatc cgctccggga
121acgagcctcg ttccgcgtcc gccatggaac ctgatggtcg cggtgcctgg gcccacagtc
181gcgccgcgct cgaccgcctg gagaagctgc tgcgctgctc gcgttgtact aacattctga
241gagagcctgt gtgtttagga ggatgtgagc acatcttctg tagtaattgt gtaagtgact
301gcattggaac tggatgtcca gtgtgttaca ccccggcctg gatacaagac ttgaagataa
361atagacaact ggacagcatg attcaacttt gtagtaagct tcgaaatttg ctacatgaca
421atgagctgtc agatttgaaa gaagataaac ctaggaaaag tttgtttaat gatgcaggaa
481acaagaagaa ttcaattaaa atgtggttta gccctcgaag taagaaagtc agatatgttg
541tgagtaaagc ttcagtgcaa acccagcctg caataaaaaa agatgcaagt gctcagcaag
601actcatatga atttgtttcc ccaagtcctc ctgcagatgt ttctgagagg gctaaaaagg
661cttctgcaag atctggaaaa aagcaaaaaa agaaaacttt agctgaaatc aaccaaaaat
721ggaatttaga ggcagaaaaa gaagatggtg aatttgactc caaagaggaa tctaagcaaa
781agctggtatc cttctgtagc caaccatctg ttatctccag tcctcagata aatggtgaaa
841tagacttact agcaagtggc tccttgacag aatctgaatg ttttggaagt ttaactgaag
901tctctttacc attggctgag caaatagagt ctccagacac taagagcagg aatgaagtag
961tgactcctga gaaggtctgc aaaaattatc ttacatctaa gaaatctttg ccattagaaa
1021ataatggaaa acgtggccat cacaatagac tttccagtcc catttctaag agatgtagaa
1081ccagcattct gagcaccagt ggagattttg ttaagcaaac ggtgccctca gaaaatatac
1141cattgcctga atgttcttca ccaccttcat gcaaacgtaa agttggtggt acatcaggga
1201gcaaaaacag taacatgtcc gatgaattca ttagtctttc accaggtaca ccaccttcta
1261cattaagtag ttcaagttac aggcgagtga tgtctagtcc ctcagcaatg aagctgttgc
1321ccaatatggc tgtgaaaaga aatcatagag gagagacttt gctccatatt gcttctatta
1381agggcgacat accttctgtt gaataccttt tacaaaatgg aagtgatcca aatgttaaag
1441accatgctgg atggacacca ttgcatgaag cttgcaatca tgggcacctg aaggtagtgg
1501aattattgct ccagcataag gcattggtga acaccaccgg gtatcaaaat gactcaccac
1561ttcacgatgc agccaagaat gggcacatgg atatagtcaa gctgttactt tcctatggag
1621cctccagaaa tgctgttaat atatttggtc tgcggcctgt cgattataca gatgatgaaa
1681gtatgaaatc gctattgctg ctaccagaga agaatgaatc atcctcagct agccactgct
1741cagtaatgaa cactgggcag cgtagggatg gacctcttgt acttataggc agtgggctgt
1801cttcagaaca acagaaaatg ctcagtgagc ttgcagtaat tcttaaggct aaaaaatata
1861ctgagtttga cagtacagta actcatgttg ttgttcctgg tgatgcagtt caaagtacct
1921tgaagtgtat gcttgggatt ctcaatggat gctggattct aaaatttgaa tgggtaaaag
1981catgtctacg aagaaaagta tgtgaacagg aagaaaagta tgaaattcct gaaggtccac
2041gcagaagcag gctcaacaga gaacagctgt tgccaaagct gtttgatgga tgctacttct
2101atttgtgggg aaccttcaaa caccatccaa aggacaacct tattaagctc gtcactgcag
2161gtgggggcca gatcctcagt agaaagccca agccagacag tgacgtgact cagaccatca
2221atacagtcgc ataccatgcg agacccgatt ctgatcagcg cttctgcaca cagtatatca
2281tctatgaaga tttgtgtaat tatcacccag agagggttcg gcagggcaaa gtctggaagg
2341ctccttcgag ctggtttata gactgtgtga tgtcctttga gttgcttcct cttgacagct
2401nnccaacttt cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca
2461acgaac

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