Construct: ORF ccsbBroadEn_15388
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004993.1_s300c1, BRDN0000395029
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CRK (1398)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1398 | CRK | CRK proto-oncogene, adaptor... | NM_005206.5 | 100% | 100% | |
| 2 | human | 1398 | CRK | CRK proto-oncogene, adaptor... | NM_016823.4 | 66.9% | 66.7% | 610_612delAAC;614A>G;616_912del |
| 3 | mouse | 12928 | Crk | v-crk avian sarcoma virus C... | NM_001277219.1 | 94.6% | 99.5% | (many diffs) |
| 4 | mouse | 12928 | Crk | v-crk avian sarcoma virus C... | XM_006532125.2 | 73.8% | 77% | (many diffs) |
| 5 | mouse | 12928 | Crk | v-crk avian sarcoma virus C... | XM_006532124.2 | 73.2% | 76.8% | (many diffs) |
| 6 | mouse | 12928 | Crk | v-crk avian sarcoma virus C... | NM_133656.5 | 63.3% | 66.4% | (many diffs) |
| 7 | mouse | 12928 | Crk | v-crk avian sarcoma virus C... | NM_001277221.1 | 37.5% | 39.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 678
- ORF length:
- 612
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag 61 ttggcatggc gggcaacttc gactcggagg agcggagtag ctggtactgg gggaggttga 121 gtcggcagga ggcggtggcg ctgctgcagg gccagcggca cggggtgttc ctggtgcggg 181 actcgagcac cagccccggg gactatgtgc tcagcgtctc agagaactcg cgcgtctccc 241 actacatcat caacagcagc ggcccgcgcc cgccggtgcc accgtcgccc gcccagcctc 301 cgcccggggt gagcccctcc agactccgaa taggagatca agagtttgat tcattgcctg 361 ctttactgga attctacaaa atacactatt tggacactac aacgttgata gaaccagttt 421 ccagatccag gcagggtagt ggagtgattc tcaggcagga ggaggcggag tatgtgcgag 481 ccctctttga ctttaatggg aatgatgagg aagatcttcc ctttaagaaa ggagacatct 541 tgagaatccg ggacaagcct gaagagcagt ggtggaatgc ggaggacagc gaaggcaaga 601 gagggatgat tccagtccct tacgtcgaga agtatagacc tgcctccgcc tcagtatcgg 661 ctctgattgg aggtcggtgc ccaactttct tgtacaaagt tggcattata agaaagcatt 721 gcttatcaat ttgttgcaac gaac