Construct: ORF ccsbBroadEn_15520

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006980.1_s300c1, BRDN0000398476
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PDHA2 (5161)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5161 PDHA2 pyruvate dehydrogenase E1 a... NM_005390.5 100% 100%
2 human 5160 PDHA1 pyruvate dehydrogenase E1 a... NM_000284.4 83.1% 85.1% (many diffs)
3 human 5160 PDHA1 pyruvate dehydrogenase E1 a... NM_001173455.1 81.6% 83.6% (many diffs)
4 human 5160 PDHA1 pyruvate dehydrogenase E1 a... NM_001173454.1 76.5% 77.6% (many diffs)
5 human 5160 PDHA1 pyruvate dehydrogenase E1 a... NM_001173456.1 76.5% 78.2% (many diffs)
6 human 5160 PDHA1 pyruvate dehydrogenase E1 a... XM_017029574.2 70.5% 71.3% (many diffs)
7 mouse 18597 Pdha1 pyruvate dehydrogenase E1 a... NM_008810.3 81% 85.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1233
ORF length:
1164
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag
61ttggcaccat gctggccgcc ttcatctccc gcgtgttgag gcgagttgcc cagaaatcag
121ctcgcagagt gctggtggca tcccgtaact cctcaaatga cgctacattt gaaattaaga
181aatgtgatct ttatctgttg gaagagggtc cccctgtcac tacagtgctc actagggcgg
241aggggcttaa atactacagg atgatgctga ctgttcgccg catggaattg aaggcagatc
301agctgtacaa acagaaattc attcgcggtt tctgtcacct gtgcgatggt caggaagctt
361gttgcgtggg ccttgaggcc ggcataaacc cctcggatca cgtcattaca tcctataggg
421ctcatggtgt gtgctatact cggggacttt ctgtccgatc cattctcgca gagctgacgg
481gaagaagagg aggttgtgct aaaggaaaag gaggatcgat gcatatgtat accaagaact
541tctatggggg caatggcatc gtcggtgcac agggccccct gggcgctggc attgctctgg
601cctgtaaata taaaggaaac gatgagatct gtttgacttt atatggggat ggcgctgcga
661atcaggggca gatagccgaa gctttcaata tggcagcttt atggaaatta ccttgtgttt
721tcatctgtga gaataaccta tatggaatgg gaacatctac tgagagagca gcagccagcc
781ctgattacta caagaggggc aattttatcc ctgggctaaa ggtcgatgga atggatgttc
841tgtgtgttcg tgaggcaaca aaatttgcag ctaactactg tagatctgga aaggggccca
901tactgatgga gctgcaaacc taccgttatc atggacacag tatgagtgat cctggagtca
961gttatcgtac acgagaagaa attcaggaag taagaagtaa gagggatcct ataataattc
1021tccaagatag aatggtaaac agcaagctcg ccactgtgga agaattaaag gaaattgggg
1081ctgaggtgag gaaagaaatt gatgatgctg cccagtttgc taccactgat cctgagccac
1141atttggaaga attaggccat cacatctaca gcagtgattc atcttttgaa gttcgtggtg
1201caaatccatg gatcaagttt aagtccgtca gttnnccaac tttcttgtac aaagttggca
1261ttataagaaa gcattgctta tcaatttgtt gcaacgaac

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