Transcript: Human NM_000284.4

Homo sapiens pyruvate dehydrogenase E1 alpha 1 subunit (PDHA1), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-09-28
Taxon:
Homo sapiens (human)
Gene:
PDHA1 (5160)
Length:
3349
CDS:
112..1284

Additional Resources:

NCBI RefSeq record:
NM_000284.4
NBCI Gene record:
PDHA1 (5160)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_000284.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000220891 GCCAATCAGTGGATCAAGTTT pLKO.1 1249 CDS 100% 5.625 7.875 N PDHA1 n/a
2 TRCN0000280980 GCCAATCAGTGGATCAAGTTT pLKO_005 1249 CDS 100% 5.625 7.875 N PDHA1 n/a
3 TRCN0000220890 GTGAGAATAATCGCTATGGAA pLKO.1 776 CDS 100% 3.000 4.200 N PDHA1 n/a
4 TRCN0000280981 GTGAGAATAATCGCTATGGAA pLKO_005 776 CDS 100% 3.000 4.200 N PDHA1 n/a
5 TRCN0000220889 GCTGGTAGCATCCCGTAATTT pLKO.1 180 CDS 100% 15.000 10.500 N PDHA1 n/a
6 TRCN0000280983 GCTGGTAGCATCCCGTAATTT pLKO_005 180 CDS 100% 15.000 10.500 N PDHA1 n/a
7 TRCN0000220892 CGAATGGAGTTGAAAGCAGAT pLKO.1 328 CDS 100% 4.050 2.835 N PDHA1 n/a
8 TRCN0000280982 CGAATGGAGTTGAAAGCAGAT pLKO_005 328 CDS 100% 4.050 2.835 N PDHA1 n/a
9 TRCN0000220888 CGAGAAATTCTCGCAGAGCTT pLKO.1 505 CDS 100% 2.640 1.584 N PDHA1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_000284.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01163 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_01163 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000474017 GATACTATTAATTACCGACAGACC pLX_317 44.5% 100% 100% V5 n/a
4 ccsbBroadEn_15520 pDONR223 0% 83.1% 85.1% None (many diffs) n/a
5 ccsbBroad304_15520 pLX_304 0% 83.1% 85.1% V5 (many diffs) n/a
6 TRCN0000481360 TGCTTTGCTGAATTACAAGGGGCC pLX_317 38.9% 83.1% 85.1% V5 (many diffs) n/a
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