Construct: ORF ccsbBroadEn_15524

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015744.1_s300c1, BRDN0000395466
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PFKL (5211)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5211 PFKL phosphofructokinase, liver ... NM_002626.6 99.9% 100% 366C>T
2 human 5211 PFKL phosphofructokinase, liver ... XM_005261135.3 97% 97.1% 159_227del;435C>T
3 human 5211 PFKL phosphofructokinase, liver ... XM_017028369.1 96.8% 96.5% (many diffs)
4 human 5211 PFKL phosphofructokinase, liver ... XM_005261137.4 96.2% 96.2% 0_1ins87;279C>T
5 human 5211 PFKL phosphofructokinase, liver ... XM_017028370.1 96.2% 96.2% 0_1ins87;279C>T
6 human 5211 PFKL phosphofructokinase, liver ... XM_017028372.1 96.2% 96.2% 0_1ins87;279C>T
7 human 5211 PFKL phosphofructokinase, liver ... NM_001002021.3 92.2% 91.4% (many diffs)
8 human 5211 PFKL phosphofructokinase, liver ... XM_024452085.1 92.2% 91.4% (many diffs)
9 human 5211 PFKL phosphofructokinase, liver ... XM_006724011.1 89.7% 88.9% (many diffs)
10 human 5211 PFKL phosphofructokinase, liver ... XM_017028368.1 89.7% 88.9% (many diffs)
11 human 5211 PFKL phosphofructokinase, liver ... XM_011529603.2 70.3% 67.9% (many diffs)
12 mouse 18641 Pfkl phosphofructokinase, liver,... NM_008826.4 87.1% 94.2% (many diffs)
13 mouse 18641 Pfkl phosphofructokinase, liver,... XM_017313846.1 52.2% 55.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2406
ORF length:
2340
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag
61ttggcatggc cgcggtggac ctggagaagc tgcgggcgtc gggcgcgggc aaggccatcg
121gcgtcctgac cagcggcggc gacgcgcaag gcatgaacgc tgctgtccgg gctgtgacgc
181gcatgggcat ttatgtgggt gccaaagtct tcctcatcta cgagggctat gagggcctcg
241tggagggagg tgagaacatc aagcaggcca actggctgag cgtctccaac atcatccagc
301tgggcggcac tatcattggc agcgctcgct gcaaggcctt taccaccagg gaggggcgcc
361gggcagcggc ctacaacctg gtccagcacg gcatcaccaa cctgtgcgtc atcggcgggg
421atggcagcct tacaggtgcc aacatcttcc gcagcgagtg gggcagcctg ctggaggagc
481tggtggcgga aggtaagatc tcagagacta cagcccggac ctactcgcac ctgaacatcg
541cgggcctagt gggctccatc gataacgact tctgcggcac cgacatgacc atcggcacgg
601actcggccct ccaccgcatc atggaggtca tcgatgccat caccaccact gcccagagcc
661accagaggac cttcgtgctg gaagtgatgg gccggcactg cgggtacctg gcgctggtat
721ctgcactggc ctcaggggcc gactggctgt tcatccccga ggctccaccc gaggacggct
781gggagaactt catgtgtgag aggctgggtg agactcggag ccgtgggtcc cgactgaaca
841tcatcatcat cgctgagggt gccattgacc gcaacgggaa gcccatctcg tccagctacg
901tgaaggacct ggtggttcag aggctgggct tcgacacccg tgtaactgtg ctgggccacg
961tgcagcgggg agggacgccc tctgccttcg accggatcct gagcagcaag atgggcatgg
1021aggcggtgat ggcgctgctg gaagccacgc ctgacacgcc ggcctgcgtg gtcaccctct
1081cggggaacca gtcagtgcgg ctgcccctca tggagtgcgt gcagatgacc aaggaagtgc
1141agaaagccat ggatgacaag aggtttgacg aggccaccca gctccgtggt gggagcttcg
1201agaacaactg gaacatttac aagctcctcg cccaccagaa gccccccaag gagaagtcta
1261acttctccct ggccatcctg aatgtggggg ccccggcggc tggcatgaat gcggccgtgc
1321gctcggcggt gcggaccggc atctcccatg gacacacagt atacgtggtg cacgatggct
1381tcgaaggcct agccaagggt caggtgcaag aagtaggctg gcacgacgtg gccggctggt
1441tggggcgtgg tggctccatg ctggggacca agaggaccct gcccaagggc cagctggagt
1501ccattgtgga gaacatccgc atctatggta ttcacgccct gctggtggtc ggtgggtttg
1561aggcctatga aggggtgctg cagctggtgg aggctcgcgg gcgctacgag gagctctgca
1621tcgtcatgtg tgtcatccca gccaccatca gcaacaacgt ccctggcacc gacttcagcc
1681tgggctccga cactgctgta aatgccgcca tggagagctg tgaccgcatc aaacagtctg
1741cctcggggac caagcgccgt gtgttcatcg tggagaccat ggggggttac tgtggctacc
1801tggccaccgt gactggcatt gctgtggggg ccgacgccgc ctacgtcttc gaggaccctt
1861tcaacatcca cgacttaaag gtcaacgtgg agcacatgac ggagaagatg aagacagaca
1921ttcagagggg cctggtgctg cggaacgaga agtgccatga ctactacacc acggagttcc
1981tgtacaacct gtactcatca gagggcaagg gcgtcttcga ctgcaggacc aatgtcctgg
2041gccacctgca gcagggtggc gctccaaccc cctttgaccg gaactatggg accaagctgg
2101gggtgaaggc catgctgtgg ttgtcggaga agctgcgcga ggtttaccgc aagggacggg
2161tgttcgccaa tgccccagac tcggcctgcg tgatcggcct gaagaagaag gcggtggcct
2221tcagccccgt cactgagctc aagaaagaca ctgatttcga gcaccgcatg ccacgggagc
2281agtggtggct gagcctgcgg ctcatgctga agatgctggc acaataccgc atcagtatgg
2341ccgcctacgt gtcaggggag ctggagcacg tgacccgccg caccctgagc atggacaagg
2401gcttctnncc aactttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt
2461gttgcaacga ac

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