Construct: ORF ccsbBroadEn_15637
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017209.1_s300c1, BRDN0000388941
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- APOL1 (8542)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8542 | APOL1 | apolipoprotein L1 | NM_001136540.1 | 59.7% | 59.7% | 715_1194del |
| 2 | human | 8542 | APOL1 | apolipoprotein L1 | NM_003661.4 | 59.7% | 59.7% | 715_1194del |
| 3 | human | 8542 | APOL1 | apolipoprotein L1 | NM_145343.2 | 57.4% | 57.4% | 1_48del;763_1242del |
| 4 | human | 8542 | APOL1 | apolipoprotein L1 | NM_001136541.1 | 55.2% | 55.2% | 43_44ins54;661_1140del |
| 5 | human | 8542 | APOL1 | apolipoprotein L1 | NM_001362927.2 | 55.2% | 55.2% | 43_44ins54;661_1140del |
| 6 | human | 23780 | APOL2 | apolipoprotein L2 | NM_030882.4 | 38.4% | 31.3% | (many diffs) |
| 7 | human | 23780 | APOL2 | apolipoprotein L2 | NM_145637.2 | 38.4% | 31.3% | (many diffs) |
| 8 | human | 23780 | APOL2 | apolipoprotein L2 | XM_011530074.1 | 38.4% | 31.3% | (many diffs) |
| 9 | human | 23780 | APOL2 | apolipoprotein L2 | XM_011530075.1 | 38.4% | 31.3% | (many diffs) |
| 10 | human | 23780 | APOL2 | apolipoprotein L2 | XM_011530076.3 | 38.4% | 31.3% | (many diffs) |
| 11 | human | 23780 | APOL2 | apolipoprotein L2 | XM_011530077.3 | 38.4% | 31.3% | (many diffs) |
| 12 | human | 23780 | APOL2 | apolipoprotein L2 | XM_011530078.1 | 38.4% | 31.3% | (many diffs) |
| 13 | human | 8542 | APOL1 | apolipoprotein L1 | XM_011530478.2 | 29.3% | 29.3% | 0_1ins363;352_831del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 780
- ORF length:
- 714
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag 61 ttggcatgga gggagctgct ttgctgagag tctctgtcct ctgcatctgg atgagtgcac 121 ttttccttgg tgtgggagtg agggcagagg aagctggagc gagggtgcaa caaaacgttc 181 caagtgggac agatactgga gatcctcaaa gtaagcccct cggtgactgg gctgctggca 241 ccatggaccc agagagcagt atctttattg aggatgccat taagtatttc aaggaaaaag 301 tgagcacaca gaatctgcta ctcctgctga ctgataatga ggcctggaac ggattcgtgg 361 ctgctgctga actgcccagg aatgaggcag atgagctccg taaagctctg gacaaccttg 421 caagacaaat gatcatgaaa gacaaaaact ggcacgataa aggccagcag tacagaaact 481 ggtttctgaa agagtttcct cggttgaaaa gtgagcttga ggataacata agaaggctcc 541 gtgcccttgc agatggggtt cagaaggtcc acaaaggcac caccatcgcc aatgtggtgt 601 ctggctctct cagcatttcc tctggcatcc tgaccctcgt cggcatgggt ctggcaccct 661 tcacagaggg aggcagcctt gtactcttgg aacctgggat ggagttggga atcacagccg 721 ctttgaccgg gattaccagc agtaccatgg actacggaaa gaagtggtgg acacaagcct 781 nnccaacttt cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca 841 acgaac