Construct: ORF ccsbBroadEn_15641

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009660.1_s300c1, BRDN0000396139
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
VAMP4 (8674)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 8674 VAMP4 vesicle associated membrane... NM_001185127.2 100% 100%
2 human 8674 VAMP4 vesicle associated membrane... NM_003762.5 99.2% 99.2% 114_116delAAG
3 human 8674 VAMP4 vesicle associated membrane... XM_017002713.2 70.2% 52% 114_116delAAG;217_218ins46;300_301ins77
4 human 8674 VAMP4 vesicle associated membrane... NR_033704.2 15.7% 1_205del;319_321delAAG;629_2675del
5 mouse 53330 Vamp4 vesicle-associated membrane... NM_001347125.1 93.3% 96.4% (many diffs)
6 mouse 53330 Vamp4 vesicle-associated membrane... XM_006496934.2 93.3% 96.4% (many diffs)
7 mouse 53330 Vamp4 vesicle-associated membrane... NM_016796.3 89.3% 92.1% (many diffs)
8 mouse 53330 Vamp4 vesicle-associated membrane... XM_006496936.3 89.3% 92.1% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
486
ORF length:
420
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag
61ttggcatgcc tcccaagttt aagcgccacc tcaatgatga tgatgtcaca ggttctgtga
121aaagtgaaag gagaaatctt ttggaagatg attcagatga agaagaggac ttttttctgg
181gaccatctgg accaagattt ggacctagaa atgataaaat taagcatgtt cagaatcaag
241tggatgaagt tattgatgtc atgcaagaaa atattacaaa ggtaattgag agaggggaga
301gactagatga actacaggac aaatcagaaa gcttatcgga taatgcaaca gcttttagca
361acagatccaa acaacttcga aggcaaatgt ggtggcgtgg atgcaaaata aaagccatca
421tggctttggt tgctgctatc cttttgctag tgattatcat tcttatagtc atgaaatacc
481gtacttnncc aactttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt
541gttgcaacga ac

Download FASTA (ORF) (Full)