Construct: ORF ccsbBroadEn_15746

Construct Description:

• Construct Type: ORF
• Other Identifiers: ORF008442.1_s300c1, BRDN0000395365
• DNA Barcode: None
• Epitope Tag: None
• Notes: No stop codon in insert

Originally Annotated References:

• Gene: WDFY3 (23001)

Vector Information:

• Vector Backbone: pDONR223
• Pol II Cassette 1: n/a
• Pol II Cassette 2: n/a
• Selection Marker: n/a
• Visible Reporter: n/a
• Epitope Tag: n/a

Current transcripts matched by this ORF:

	Taxon	Gene	Symbol	Description	Transcript	Nuc. Match %	Prot. Match %	Match Diffs
1	human	23001	WDFY3	WD repeat and FYVE domain c...	XM_017007909.1	9.7%	9.8%	1_7755del;8457T>C
2	human	23001	WDFY3	WD repeat and FYVE domain c...	XM_011531765.3	7.9%	8%	1_9684del;10386T>C
3	human	23001	WDFY3	WD repeat and FYVE domain c...	XM_011531764.3	7.9%	7.9%	1_9705del;10407T>C
4	human	23001	WDFY3	WD repeat and FYVE domain c...	NM_014991.5	7.9%	7.9%	1_9735del;10437T>C
5	human	23001	WDFY3	WD repeat and FYVE domain c...	XM_011531763.3	7.9%	7.9%	1_9735del;10437T>C
6	human	23001	WDFY3	WD repeat and FYVE domain c...	XM_011531762.3	7.9%	7.9%	1_9738del;10440T>C
7	human	23001	WDFY3	WD repeat and FYVE domain c...	XM_011531761.3	7.9%	7.9%	1_9744del;10446T>C
8	human	23001	WDFY3	WD repeat and FYVE domain c...	XM_005262858.5	7.9%	7.9%	1_9789del;10491T>C
9	human	23001	WDFY3	WD repeat and FYVE domain c...	XM_011531757.3	7.9%	7.9%	1_9789del;10491T>C
10	human	23001	WDFY3	WD repeat and FYVE domain c...	XM_011531759.2	7.9%	7.9%	1_9789del;10491T>C
11	human	23001	WDFY3	WD repeat and FYVE domain c...	XM_011531760.3	7.9%	7.9%	1_9789del;10491T>C
12	human	23001	WDFY3	WD repeat and FYVE domain c...	XM_017007906.2	7.9%	7.9%	1_9789del;10491T>C
13	human	23001	WDFY3	WD repeat and FYVE domain c...	XM_017007907.2	7.9%	7.9%	1_9789del;10491T>C
14	human	23001	WDFY3	WD repeat and FYVE domain c...	XM_017007908.2	7.9%	7.9%	1_9789del;10491T>C
15	mouse	72145	Wdfy3	WD repeat and FYVE domain c...	XM_006535227.3	7%	7.5%	(many diffs)
16	mouse	72145	Wdfy3	WD repeat and FYVE domain c...	NM_172882.3	6.9%	7.4%	(many diffs)
17	mouse	72145	Wdfy3	WD repeat and FYVE domain c...	XM_006535226.3	6.9%	7.4%	(many diffs)
18	mouse	72145	Wdfy3	WD repeat and FYVE domain c...	XM_006535225.3	6.9%	7.4%	(many diffs)
19	mouse	72145	Wdfy3	WD repeat and FYVE domain c...	XM_006535224.3	6.9%	7.4%	(many diffs)
20	mouse	72145	Wdfy3	WD repeat and FYVE domain c...	XM_006535220.3	6.9%	7.4%	(many diffs)
21	mouse	72145	Wdfy3	WD repeat and FYVE domain c...	XM_006535221.2	6.9%	7.4%	(many diffs)
22	mouse	72145	Wdfy3	WD repeat and FYVE domain c...	XM_006535222.3	6.9%	7.4%	(many diffs)
23	mouse	72145	Wdfy3	WD repeat and FYVE domain c...	XM_006535223.3	6.9%	7.4%	(many diffs)

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:

66

ORF end:

909

ORF length:

843

Sequence:

1	gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag
61	ttggcatgga atttttgcaa gttcctgaaa caccagctcc tgagcctgct gaagtcctag
121	aaatgcagga agactgtcca gaagcacaaa tagggcagga agcccaagac gaggacagca
181	gtgattcaga agcagatgag cagagcatca gccaggaccc taaggacact ccaagccaac
241	ccagcagcac cagccacagg ccccgggcag cctcctgccg cgcaacagcc gcctggtgta
301	ctgacagtgg ctctgacgac tccagacgct ggtccgacca gctcagtcta gatgagaaag
361	acggcttcat atttgtgaac tattcagagg gccagaccag agcccatctg cagggccccc
421	ttagccaccc ccaccccaat cccattgagg tgcggaatta cagcagattg aaacctgggt
481	accgatggga acggcagctg gtgttcagga gtaagctgac tatgcacaca gcctttgatc
541	gaaaggacaa tgcacaccca gctgaggtca ctgccttggg catctccaag gatcacagta
601	ggatcctcgt tggtgacagt cgaggccgag ttttcagctg gtctgtgagt gaccagccag
661	gccgttctgc tgctgatcac tgggtgaagg atgaaggtgg tgacagctgc tcaggctgct
721	cggtgaggtt ttcactcaca gaaagacgac accattgcag gaactgcggt cagctcttct
781	gccagaagtg cagtcgcttt caatctgaaa tcaaacgctt gaaaatctca tccccggtgc
841	gtgtttgtca gaactgttat tataacttac agcatgagag aggttcagaa gatgggcctc
901	gaaattgttn nccaactttc ttgtacaaag ttggcattat aagaaagcat tgcttatcaa
961	tttgttgcaa cgaac

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