Transcript: Mouse XM_006535221.2

PREDICTED: Mus musculus WD repeat and FYVE domain containing 3 (Wdfy3), transcript variant X2, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Wdfy3 (72145)
Length:
15358
CDS:
1570..12153

Additional Resources:

NCBI RefSeq record:
XM_006535221.2
NBCI Gene record:
Wdfy3 (72145)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006535221.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000311327 TATGGCCGACAACGCTAATTA pLKO_005 2802 CDS 100% 15.000 21.000 N Wdfy3 n/a
2 TRCN0000111646 GCGCCCACAATATAGTGAAAT pLKO.1 3293 CDS 100% 13.200 18.480 N Wdfy3 n/a
3 TRCN0000111648 GCGCAGTTACAGCAGATTGAA pLKO.1 11694 CDS 100% 5.625 7.875 N Wdfy3 n/a
4 TRCN0000351338 GCGCAGTTACAGCAGATTGAA pLKO_005 11694 CDS 100% 5.625 7.875 N Wdfy3 n/a
5 TRCN0000111649 CGCCACTACTTTACAGTACAT pLKO.1 5772 CDS 100% 4.950 3.960 N Wdfy3 n/a
6 TRCN0000305435 CTCGCTCACTGCCTGATTAAT pLKO_005 7999 CDS 100% 15.000 10.500 N Wdfy3 n/a
7 TRCN0000305491 TAGAGCTATTCATCCTTATTT pLKO_005 12452 3UTR 100% 15.000 10.500 N Wdfy3 n/a
8 TRCN0000305492 TTCTAGCGTGACTACTATTTA pLKO_005 5400 CDS 100% 15.000 10.500 N Wdfy3 n/a
9 TRCN0000111647 GCCAAGTTGATGAGGGAATTT pLKO.1 6178 CDS 100% 13.200 9.240 N Wdfy3 n/a
10 TRCN0000111645 GCTGAGTGTAAAGGAATCATT pLKO.1 12267 3UTR 100% 5.625 3.938 N Wdfy3 n/a
11 TRCN0000015008 CCAACCAACCAACCAACCAAA pLKO.1 898 5UTR 100% 4.950 2.475 Y HMBOX1 n/a
12 TRCN0000240702 CGGATATGGCAAGGATATAAT pLKO_005 2482 CDS 100% 15.000 21.000 N WDFY3 n/a
13 TRCN0000166364 CACACACACACACACACACAA pLKO.1 228 5UTR 100% 4.950 2.475 Y KAAG1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006535221.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11658 pDONR223 100% 10.4% 11.3% None (many diffs) n/a
2 ccsbBroad304_11658 pLX_304 0% 10.4% 11.3% V5 (many diffs) n/a
3 TRCN0000481278 ATGTTAGGGCATGGGCCGAAACAA pLX_317 37.5% 10.4% 11.3% V5 (many diffs) n/a
4 ccsbBroadEn_15746 pDONR223 0% 6.9% 7.4% None (many diffs) n/a
5 ccsbBroad304_15746 pLX_304 0% 6.9% 7.4% V5 (many diffs) n/a
6 TRCN0000481357 CCGACATATTCGCACACAATTTTG pLX_317 47.9% 6.9% 7.4% V5 (many diffs) n/a
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