Construct: ORF ccsbBroadEn_15763

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018028.1_s300c1, BRDN0000397324
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ISCU (23479)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23479 ISCU iron-sulfur cluster assembl... NM_213595.3 100% 100%
2 human 23479 ISCU iron-sulfur cluster assembl... NM_001301140.1 85.6% 85.2% (many diffs)
3 human 23479 ISCU iron-sulfur cluster assembl... NM_001320042.1 85.6% 85.2% (many diffs)
4 human 23479 ISCU iron-sulfur cluster assembl... NM_001301141.1 85.2% 82.9% (many diffs)
5 human 23479 ISCU iron-sulfur cluster assembl... NM_014301.4 83.1% 77.8% (many diffs)
6 human 23479 ISCU iron-sulfur cluster assembl... XM_011538101.2 76.6% 66.4% 339_340ins79;384_385ins38
7 human 23479 ISCU iron-sulfur cluster assembl... XM_011538100.2 69.4% 63.3% (many diffs)
8 human 23479 ISCU iron-sulfur cluster assembl... NR_135127.1 21.3% (many diffs)
9 mouse 66383 Iscu iron-sulfur cluster assembl... NM_025526.4 87.7% 95.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
567
ORF length:
501
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag
61ttggcatggc ggcggctggg gctttccgtc tgaggcgggc ggcatcggct ctgctgctgc
121ggagcccccg cctgcccgcc cgggagctgt cggccccggc ccgactctat cacaagaagg
181ttgttgatca ttatgaaaat cctagaaacg tggggtccct tgacaagaca tctaaaaatg
241ttggaactgg actggtgggg gctccagcat gtggtgacgt aatgaaatta cagattcaag
301tggatgaaaa ggggaagatt gtggatgcta ggtttaaaac atttggctgt ggttccgcaa
361ttgcctccag ctcattagcc actgaatggg tgaaaggaaa gacggtggag gaagccttga
421ctatcaaaaa cacagatatc gccaaggagc tctgccttcc tcccgtgaaa ctgcactgct
481ccatgctggc tgaagatgca atcaaggccg ccctggctga ttacaaattg aaacaagaac
541ccaaaaaagg agaggcagag aagaaatnnc caactttctt gtacaaagtt ggcattataa
601gaaagcattg cttatcaatt tgttgcaacg aac

Download FASTA (ORF) (Full)