Construct: ORF ccsbBroadEn_16060
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011769.1_s300c1, BRDN0000395308
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SPSB3 (90864)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 90864 | SPSB3 | splA/ryanodine receptor dom... | NM_001324081.1 | 63.8% | 63.9% | 1_384del;633A>G |
| 2 | human | 90864 | SPSB3 | splA/ryanodine receptor dom... | NM_080861.4 | 63.8% | 63.9% | 1_384del;633A>G |
| 3 | human | 90864 | SPSB3 | splA/ryanodine receptor dom... | XM_005255673.5 | 55.2% | 55.3% | 1_549del;798A>G |
| 4 | mouse | 79043 | Spsb3 | splA/ryanodine receptor dom... | XM_006525170.3 | 49.7% | 52.8% | (many diffs) |
| 5 | mouse | 79043 | Spsb3 | splA/ryanodine receptor dom... | XM_011246733.2 | 49.7% | 52.8% | (many diffs) |
| 6 | mouse | 79043 | Spsb3 | splA/ryanodine receptor dom... | NM_001163751.1 | 48.8% | 52% | (many diffs) |
| 7 | mouse | 79043 | Spsb3 | splA/ryanodine receptor dom... | NM_027141.2 | 48.3% | 51.5% | (many diffs) |
| 8 | mouse | 79043 | Spsb3 | splA/ryanodine receptor dom... | XM_006525169.3 | 46% | 48.9% | (many diffs) |
| 9 | mouse | 79043 | Spsb3 | splA/ryanodine receptor dom... | XM_006525168.3 | 45.6% | 48.4% | (many diffs) |
| 10 | mouse | 79043 | Spsb3 | splA/ryanodine receptor dom... | NM_001163750.1 | 37.6% | 40.1% | (many diffs) |
| 11 | mouse | 79043 | Spsb3 | splA/ryanodine receptor dom... | XM_006525165.3 | 36% | 38.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 750
- ORF length:
- 681
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag 61 ttggcaccat ggagtacagc tgcggcacag cggccatccg gggcaccaag gagctggggg 121 agggccagca cttctgggag atcaagatga cctctcccgt ctacggcacc gacatgatgg 181 tgggcatcgg gacgtcggat gtggacctgg acaaataccg ccacacgttc tgcagcctgc 241 tgggcaggga tgaggacagc tggggcctct cctacacggg cctcctccac cacaagggcg 301 acaagaccag cttctcgtcg cggttcggcc agggctccat cattggcgtg cacctggaca 361 cctggcacgg cacactcacc tttttcaaga acaggaagtg tataggtgtg gcagccacca 421 agctgcagaa caagagattc tacccgatgg tgtgctccac ggcggcccgg agcagcatga 481 aggtcacccg ctcctgtgcc agcgccactt ccctccagta cctgtgctgc caccgcctgc 541 gccagctgcg gccagactcg ggagacacgc tggagggtct gccgctgccg ccgggcctca 601 agcaggtgct acacaacaag ctgggctggg tcctgagcat gagttgcagc cgccgcaagg 661 ctccagtgtc cgatccccag gcagcgacct ccgcccaccc cagcagtcgc gagcctcggc 721 cctgccagag gaagcgctgc cgccggacct tgccaacttt cttgtacaaa gttggcatta 781 taagaaagca ttgcttatca atttgttgca acgaac