Construct: ORF ccsbBroadEn_16098
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014135.1_s300c1, BRDN0000395024
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PSMA8 (143471)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 143471 | PSMA8 | proteasome 20S subunit alpha 8 | NM_001025097.2 | 93.2% | 92.9% | 503A>G;595_636del |
| 2 | human | 143471 | PSMA8 | proteasome 20S subunit alpha 8 | NM_001025096.2 | 79% | 78.8% | 114_227del;617A>G;709_750del |
| 3 | human | 143471 | PSMA8 | proteasome 20S subunit alpha 8 | NM_001308188.2 | 77.3% | 68.2% | (many diffs) |
| 4 | human | 143471 | PSMA8 | proteasome 20S subunit alpha 8 | NM_144662.3 | 77.2% | 76.9% | 114_245del;635A>G;727_768del |
| 5 | human | 143471 | PSMA8 | proteasome 20S subunit alpha 8 | XM_011525818.1 | 75.3% | 63.1% | (many diffs) |
| 6 | mouse | 73677 | Psma8 | proteasome (prosome, macrop... | NM_001163609.1 | 70% | 74.8% | (many diffs) |
| 7 | mouse | 73677 | Psma8 | proteasome (prosome, macrop... | XM_006526294.3 | 67.3% | 70.2% | (many diffs) |
| 8 | mouse | 73677 | Psma8 | proteasome (prosome, macrop... | XM_006526293.2 | 55.7% | 60% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 660
- ORF length:
- 594
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag 61 ttggcatggc gtctcgatat gacagggcga tcactgtctt ctccccagac ggacaccttt 121 ttcaagttga atatgcccag gaagcggtga agaaaggatc caccgcggtc ggaattcgag 181 gacttactgc tgatgctaga gtagtaataa acagagcccg tgtggagtgc cagagccata 241 agcttacggt tgaggaccca gtcactgtag aatacataac tcgcttcata gcaactttaa 301 agcagaaata tacccaaagc aatggacgaa gaccttttgg tatttctgcc ttaattgtag 361 gttttgatga tgatggtatc tcaagattgt atcagacaga tccttctggt acttatcatg 421 cttggaaggc aaatgcaata ggccgaagtg ctaaaactgt tcgagaattt ctagaaaaga 481 attacacaga agatgccata gcaagtgaca gtgaagctat caagttagca ataaaagctt 541 tgctagaagt tgtccagtct ggtggaagaa acattgaact tgctataata agaagaaatc 601 aacctttgaa gatgtttagt gcaaaagaag ttgaattata tgtaactgaa atagaaaagt 661 acccaacttt cttgtacaaa gttggcatta taagaaagca ttgcttatca atttgttgca 721 acgaac