Transcript: Human NM_001308188.2

Homo sapiens proteasome 20S subunit alpha 8 (PSMA8), transcript variant 4, mRNA.

Source:
NCBI, updated 2019-08-30
Taxon:
Homo sapiens (human)
Gene:
PSMA8 (143471)
Length:
1859
CDS:
241..915

Additional Resources:

NCBI RefSeq record:
NM_001308188.2
NBCI Gene record:
PSMA8 (143471)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001308188.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000413317 ATGACCACTGGGAGGTCTTAA pLKO_005 924 3UTR 100% 13.200 18.480 N PSMA8 n/a
2 TRCN0000429605 ACAGTGAAGCTATCAAGTTAG pLKO_005 719 CDS 100% 10.800 15.120 N PSMA8 n/a
3 TRCN0000414508 ACTCGCTTCATAGCAACTTTA pLKO_005 490 CDS 100% 13.200 10.560 N PSMA8 n/a
4 TRCN0000436037 TGTACTGCCTGAGGTTGTTTA pLKO_005 958 3UTR 100% 13.200 9.240 N PSMA8 n/a
5 TRCN0000046844 CAGAAATATACCCAAAGCAAT pLKO.1 514 CDS 100% 4.950 3.465 N PSMA8 n/a
6 TRCN0000046845 CTTCTGGTACTTATCATGCTT pLKO.1 614 CDS 100% 3.000 2.100 N PSMA8 n/a
7 TRCN0000046846 GTAGTAATAAACAGAGCCCGT pLKO.1 412 CDS 100% 0.540 0.378 N PSMA8 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001308188.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14389 pDONR223 100% 81.5% 79.6% None (many diffs) n/a
2 ccsbBroad304_14389 pLX_304 0% 81.5% 79.6% V5 (many diffs) n/a
3 TRCN0000474962 CCTTGCCCGCTCACACCCGCCATT pLX_317 70.5% 81.5% 79.6% V5 (many diffs) n/a
4 ccsbBroadEn_16098 pDONR223 0% 77.3% 68.2% None (many diffs) n/a
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