Gene: Human TYMS (7298)
thymidylate synthetase
- Source:
- NCBI, updated 2019-09-16
- Taxon:
- Homo sapiens (human)
- Chromosome:
- 18
- Wildtype Transcripts:
- NM_001071.4, NM_001354867.2, NM_001354868.2, XM_024451242.1
sgRNA constructs originally intended to target this gene (CRISPRko, NGG PAM)
NOTE: This table includes all CRISPRko sgRNA constructs that were intended to target this gene at the time they were designed. Because the quality and coverage of genomic reference data evolves over time, one or more of these constructs may no longer be considered a 100% sequence match for this gene. Check the "Matching Genes" column to verify the sequence match with this and any other genes in this taxon. To discover constructs that are sequence matches to current transcripts for this gene, click on the individual wild-type transcripts listed above.
shRNA constructs with 100% match to this gene
Matching is performed using the Specificity-Defining Region (SDR)[?]The SDR or "Specificity-Defining Region" encompasses the 19 bases within the shRNA stem region that are retained during siRNA processing/production. These are thus the bases that define the target specificity of the shRNA. Note that, while our shRNA designs nearly always extend the stem by two additional bases matching the intended target transcript (reported in the "Target Seq" column), these additional bases are not relevant to target specificity. of the shRNAs. This list includes matches to any current transcript from gene 7298 (TYMS), regardless of what transcript the shRNAs were originally designed to target. For example, some shRNAs in this list may have been originally designed to target: (i) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (ii) a transcript of a different gene from the same or different taxon.
shRNA constructs with at least a near match to this gene
This list includes shRNAs that have a >84% (16 of 19 bases) SDR[?]The SDR or "Specificity-Defining Region" encompasses the 19 bases within the shRNA stem region that are retained during siRNA processing/production. These are thus the bases that define the target specificity of the shRNA. Note that, while our shRNA designs nearly always extend the stem by two additional bases matching the intended target transcript (reported in the "Target Seq" column), these additional bases are not relevant to target specificity.match to transcripts from gene 7298 (TYMS), regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (ii) a transcript of a different gene from the same or different taxon.