Post-Screen Analysis
When reviewing screening data, a library .chip file will be a useful resource.
How Do I Pick a .chip File
from the GPP provides a walkthrough of what a .chip file is and how to pick the correct one.
GPP has developed a web tool, Apron Beta , to assist with the analysis of pooled screens. Apron currently supports analysis of CRISPRko, CRISPRa, and CRISPRi screens conducted with either Cas9 or Cas12a, so long as each construct targets a single gene, regardless of the number of guides in each construct targeting that gene. The accompanying Pooled Screen Analysis with Apron user guide provides step-by-step instructions as well as suggestions for how to approach screen analysis.
Base editing screen analysis guidance is described separately in our Base Editing Data Analysis Guide.
FAQs:
Q: How does GPP deconvolute sequencing data?
GPP uses an internal software tool, PoolQ, to deconvolute and quantitate perturbation barcodes in multiplexed sequencing data. For more detail on how PoolQ works, review the information on the PoolQ Software Tool Downloads page.
Q: Is there additional information available on screen analysis?
For further reading on conducting screen analysis, please see Design and analysis of CRISPR-Cas experiments.