Transcript: Human NM_001024660.4

Homo sapiens kalirin RhoGEF kinase (KALRN), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-08-06
Taxon:
Homo sapiens (human)
Gene:
KALRN (8997)
Length:
15982
CDS:
158..9118

Additional Resources:

NCBI RefSeq record:
NM_001024660.4
NBCI Gene record:
KALRN (8997)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001145005 GCAGAATACGTACACCAATG pXPR_003 CGG 1777 20% 11 0.7084 KALRN KALRN 77639
2 BRDN0001146234 AAGCAGAAGAAAGTTCGCGA pXPR_003 TGG 5381 60% 35 0.5438 KALRN KALRN 77637
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001024660.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000234366 GCGGAAACTCGTGACGTATTT pLKO_005 379 CDS 100% 13.200 18.480 N KALRN n/a
2 TRCN0000196681 GTTGCACATTTGGACATAAAG pLKO.1 8552 CDS 100% 13.200 18.480 N KALRN n/a
3 TRCN0000048211 GCTCAGAATACGTACACCAAT pLKO.1 1916 CDS 100% 4.950 6.930 N KALRN n/a
4 TRCN0000001427 GCACAATTAGATGACAAGTTT pLKO.1 9727 3UTR 100% 5.625 4.500 N KALRN n/a
5 TRCN0000048208 CCCGGAAGAAAGAATTTATTA pLKO.1 3993 CDS 100% 15.000 10.500 N KALRN n/a
6 TRCN0000234368 CTTCAGGACACACGAAATATG pLKO_005 4698 CDS 100% 13.200 9.240 N KALRN n/a
7 TRCN0000001428 GCAAAGATGTGGCTGTGAAAT pLKO.1 8277 CDS 100% 13.200 9.240 N KALRN n/a
8 TRCN0000196857 GTGATGATCTTGACCCTAATA pLKO.1 7503 CDS 100% 13.200 9.240 N KALRN n/a
9 TRCN0000001431 CAACGTATGCAGGGTGGATTT pLKO.1 8827 CDS 100% 10.800 7.560 N KALRN n/a
10 TRCN0000234369 TGATTCAAGAAAGGATCATTC pLKO_005 4890 CDS 100% 10.800 7.560 N KALRN n/a
11 TRCN0000001430 CAAAGATTACTATGCACTGAA pLKO.1 7144 CDS 100% 4.950 3.465 N KALRN n/a
12 TRCN0000048210 CCTGTCCAAAGGATCACCAAA pLKO.1 4397 CDS 100% 4.950 3.465 N KALRN n/a
13 TRCN0000048212 GCATATCATCTTTGGCAACAT pLKO.1 4135 CDS 100% 4.950 3.465 N KALRN n/a
14 TRCN0000048209 GCCATGAACAACATGACCTTT pLKO.1 2615 CDS 100% 4.950 3.465 N KALRN n/a
15 TRCN0000001429 GTGGAGTTAATGTGCCTTGTT pLKO.1 6428 CDS 100% 4.950 3.465 N KALRN n/a
16 TRCN0000026869 GCCACCATTTCTTGGAAGGAA pLKO.1 8171 CDS 100% 3.000 2.100 N LOC385625 n/a
17 TRCN0000195291 CTGATAGTTCTCTGAACTAAA pLKO.1 10323 3UTR 100% 1.320 0.924 N KALRN n/a
18 TRCN0000195470 CCAATGTCAAGAGCTACATTG pLKO.1 9072 CDS 100% 1.080 0.756 N KALRN n/a
19 TRCN0000234367 GGACCTGGAGCTGGATATTAT pLKO_005 3895 CDS 100% 15.000 9.000 N KALRN n/a
20 TRCN0000024368 GTTCTGACATATGTCATGCTT pLKO.1 8759 CDS 100% 0.000 0.000 N Kalrn n/a
21 TRCN0000026939 CCACATCCTACATCCTGATTT pLKO.1 8406 CDS 100% 13.200 9.240 N LOC385625 n/a
22 TRCN0000166201 CATGGTGAAACCCTGTCTCTA pLKO.1 11419 3UTR 100% 4.950 2.475 Y ORAI2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001024660.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

No results found.