Transcript: Human NM_001177310.1

Homo sapiens transient receptor potential cation channel subfamily M member 6 (TRPM6), transcript variant b, mRNA.

Source:
NCBI, updated 2019-08-13
Taxon:
Homo sapiens (human)
Gene:
TRPM6 (140803)
Length:
8250
CDS:
73..6126

Additional Resources:

NCBI RefSeq record:
NM_001177310.1
NBCI Gene record:
TRPM6 (140803)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001145550 GGAGCGTGGATAATAACTGA pXPR_003 AGG 512 8% 5 0.8926 TRPM6 TRPM6 77337
2 BRDN0001145361 TCTGGAATAGGCTCAAGACA pXPR_003 AGG 833 14% 8 0.702 TRPM6 TRPM6 77335
3 BRDN0001144989 AGGTCATGATGTAGCGATAG pXPR_003 CGG 3251 54% 24 0.4583 TRPM6 TRPM6 77334
4 BRDN0001148109 GGATGGTAAGAAAGCGATGG pXPR_003 AGG 1385 23% 12 0.3131 TRPM6 TRPM6 77336
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001177310.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000199953 GCGGCAATGATCCAGGTATTG pLKO.1 5347 CDS 100% 10.800 15.120 N TRPM6 n/a
2 TRCN0000199682 GCGGGAAATTTGGGTGATTTG pLKO.1 7993 3UTR 100% 10.800 15.120 N TRPM6 n/a
3 TRCN0000021587 CCAAATTCTAATGGAGTGTAT pLKO.1 1155 CDS 100% 4.950 6.930 N TRPM6 n/a
4 TRCN0000199969 CCTCATTGGTAGAGCATATCG pLKO.1 1614 CDS 100% 4.950 6.930 N TRPM6 n/a
5 TRCN0000021588 GCTCCCTATCTGATAACTCAA pLKO.1 4547 CDS 100% 4.950 6.930 N TRPM6 n/a
6 TRCN0000021585 CCCTCTAATCTAAAGCGAGTT pLKO.1 4099 CDS 100% 4.050 5.670 N TRPM6 n/a
7 TRCN0000196800 GACAGATCCATCTGTTATAAA pLKO.1 5850 CDS 100% 1.500 2.100 N TRPM6 n/a
8 TRCN0000197023 GAGTTCCACAAGGGCTAATTT pLKO.1 7937 3UTR 100% 15.000 12.000 N TRPM6 n/a
9 TRCN0000021584 CCTGGCATAAAGAATGTATAT pLKO.1 7486 3UTR 100% 13.200 9.240 N TRPM6 n/a
10 TRCN0000021586 CCGGAAGTATAACAACAACAA pLKO.1 5706 CDS 100% 4.950 3.465 N TRPM6 n/a
11 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 7176 3UTR 100% 5.625 2.813 Y KLHL30 n/a
12 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 7176 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001177310.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

No results found.