Transcript: Human NM_001257230.2

Homo sapiens ALG13 UDP-N-acetylglucosaminyltransferase subunit (ALG13), transcript variant 5, mRNA.

Source:
NCBI, updated 2019-08-08
Taxon:
Homo sapiens (human)
Gene:
ALG13 (79868)
Length:
3772
CDS:
258..3122

Additional Resources:

NCBI RefSeq record:
NM_001257230.2
NBCI Gene record:
ALG13 (79868)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001257230.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000236275 AGCCACTCGTAGTGGTTATAA pLKO_005 226 5UTR 100% 15.000 21.000 N ALG13 n/a
2 TRCN0000034823 GCCACTCGTAGTGGTTATAAA pLKO.1 227 5UTR 100% 15.000 21.000 N ALG13 n/a
3 TRCN0000183525 GCTTTATATTAGCTGATGGTA pLKO.1 3396 3UTR 100% 3.000 2.400 N ALG13 n/a
4 TRCN0000183416 CTCAAATTCATGGTGCTATAA pLKO.1 3034 CDS 100% 13.200 9.240 N ALG13 n/a
5 TRCN0000181004 GCAGCGGCTAATCAAGCTATT pLKO.1 2547 CDS 100% 10.800 7.560 N ALG13 n/a
6 TRCN0000034822 ACCAGCTTTGACGACCTCATT pLKO.1 80 5UTR 100% 4.950 3.465 N ALG13 n/a
7 TRCN0000179842 CCAGTAATGTCAGATCCCTAT pLKO.1 2910 CDS 100% 4.050 2.835 N ALG13 n/a
8 TRCN0000180918 GCAGTGCTATTACCACAGCTA pLKO.1 2678 CDS 100% 2.640 1.848 N ALG13 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001257230.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10253 pDONR223 100% 48.1% 48.1% None 1_1485del n/a
2 ccsbBroad304_10253 pLX_304 0% 48.1% 48.1% V5 1_1485del n/a
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