Transcript: Human NM_001258429.2

Homo sapiens chromosome 20 open reading frame 27 (C20orf27), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-08-09
Taxon:
Homo sapiens (human)
Gene:
C20orf27 (54976)
Length:
1252
CDS:
178..702

Additional Resources:

NCBI RefSeq record:
NM_001258429.2
NBCI Gene record:
C20orf27 (54976)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001258429.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000263509 GAAATGGGTTGAGCCCGAAAG pLKO_005 931 3UTR 100% 6.000 8.400 N C20orf27 n/a
2 TRCN0000263506 TGTGAGTACTCGGCGCACAAA pLKO_005 493 CDS 100% 4.950 6.930 N C20orf27 n/a
3 TRCN0000263505 TCCTGCACAGGTATGAGATTA pLKO_005 356 CDS 100% 13.200 9.240 N C20orf27 n/a
4 TRCN0000264711 TCCTGCACAGGTATGAGATTA pLKO_005 356 CDS 100% 13.200 9.240 N 1700037H04Rik n/a
5 TRCN0000263507 GCGTCCTCAAAGAGGAGATAC pLKO_005 518 CDS 100% 10.800 7.560 N C20orf27 n/a
6 TRCN0000263508 CCCGTCCCTGAAGGTTATAGT pLKO_005 466 CDS 100% 5.625 3.938 N C20orf27 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001258429.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12128 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_12128 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000481494 TCCAACACATGATGACATGTCGAA pLX_317 88.1% 100% 100% V5 n/a
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