Construct: ORF TRCN0000481494

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005218.1_s317c1
Derived from:
ccsbBroadEn_12128
DNA Barcode:
TCCAACACATGATGACATGTCGAA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
C20orf27 (54976)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000481494
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 54976 C20orf27 chromosome 20 open reading ... NM_001258429.2 100% 100%
2 human 54976 C20orf27 chromosome 20 open reading ... NM_001258430.2 100% 100%
3 human 54976 C20orf27 chromosome 20 open reading ... NM_001039140.3 87.4% 87.4% 19_93del
4 human 54976 C20orf27 chromosome 20 open reading ... XM_011529266.3 66.8% 66.8% 19_93del;375_376ins123
5 human 54976 C20orf27 chromosome 20 open reading ... NR_047675.1 35.8% 1_373del;896_1457del
6 mouse 67326 1700037H04Rik RIKEN cDNA 1700037H04 gene NM_026091.2 88.3% 90.2% (many diffs)
7 mouse 67326 1700037H04Rik RIKEN cDNA 1700037H04 gene XM_011239745.1 88.3% 90.2% (many diffs)
8 mouse 67326 1700037H04Rik RIKEN cDNA 1700037H04 gene NM_001311138.1 87.1% 88.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
588
ORF length:
522
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc tgcagccaac aagggcaaca agcccagagt ccggagtatc cgctttgcgg
121CAGGCCACGA TGCAGAAGGA TCCCACAGCC ACGTCCACTT TGATGAGAAG CTGCATGACT
181CGGTGGTCAT GGTCACCCAG GAGAGTGACA GCAGCTTTCT GGTCAAGGTT GGCTTCCTGA
241AGATCCTGCA CAGGTATGAG ATTACCTTCA CTCTGCCCCC AGTGCACAGG CTGAGCAAGG
301ATGTCCGCGA GGCACCTGTC CCCAGCCTGC ACCTCAAGCT CCTCAGCGTG GTGCCCGTCC
361CTGAAGGTTA TAGTGTCAAG TGTGAGTACT CGGCGCACAA AGAGGGCGTC CTCAAAGAGG
421AGATACTGCT AGCCTGCGAA GGTGGCACTG GCACCTGTGT GCGCGTGACG GTGCAGGCCC
481GCGTCATGGA CCGGCACCAC GGCACGCCCA TGCTGCTGGA TGGTGTCAAG TGTGTGGGCG
541CCGAGCTGGA ATACGACTCA GAGCACAGCG ACTGGCACGG CTTTGACTGC CCAACTTTCT
601TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA
661CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT
721GTGGAAAGGA CGATCCAACA CATGATGACA TGTCGAAACG CGTTAAGTCg acaatcaacc
781tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)