Construct: ORF TRCN0000481494
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005218.1_s317c1
- Derived from:
- ccsbBroadEn_12128
- DNA Barcode:
- TCCAACACATGATGACATGTCGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C20orf27 (54976)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000481494
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54976 | C20orf27 | chromosome 20 open reading ... | NM_001258429.2 | 100% | 100% | |
| 2 | human | 54976 | C20orf27 | chromosome 20 open reading ... | NM_001258430.2 | 100% | 100% | |
| 3 | human | 54976 | C20orf27 | chromosome 20 open reading ... | NM_001039140.3 | 87.4% | 87.4% | 19_93del |
| 4 | human | 54976 | C20orf27 | chromosome 20 open reading ... | XM_011529266.3 | 66.8% | 66.8% | 19_93del;375_376ins123 |
| 5 | human | 54976 | C20orf27 | chromosome 20 open reading ... | NR_047675.1 | 35.8% | 1_373del;896_1457del | |
| 6 | mouse | 67326 | 1700037H04Rik | RIKEN cDNA 1700037H04 gene | NM_026091.2 | 88.3% | 90.2% | (many diffs) |
| 7 | mouse | 67326 | 1700037H04Rik | RIKEN cDNA 1700037H04 gene | XM_011239745.1 | 88.3% | 90.2% | (many diffs) |
| 8 | mouse | 67326 | 1700037H04Rik | RIKEN cDNA 1700037H04 gene | NM_001311138.1 | 87.1% | 88.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 588
- ORF length:
- 522
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgcagccaac aagggcaaca agcccagagt ccggagtatc cgctttgcgg 121 CAGGCCACGA TGCAGAAGGA TCCCACAGCC ACGTCCACTT TGATGAGAAG CTGCATGACT 181 CGGTGGTCAT GGTCACCCAG GAGAGTGACA GCAGCTTTCT GGTCAAGGTT GGCTTCCTGA 241 AGATCCTGCA CAGGTATGAG ATTACCTTCA CTCTGCCCCC AGTGCACAGG CTGAGCAAGG 301 ATGTCCGCGA GGCACCTGTC CCCAGCCTGC ACCTCAAGCT CCTCAGCGTG GTGCCCGTCC 361 CTGAAGGTTA TAGTGTCAAG TGTGAGTACT CGGCGCACAA AGAGGGCGTC CTCAAAGAGG 421 AGATACTGCT AGCCTGCGAA GGTGGCACTG GCACCTGTGT GCGCGTGACG GTGCAGGCCC 481 GCGTCATGGA CCGGCACCAC GGCACGCCCA TGCTGCTGGA TGGTGTCAAG TGTGTGGGCG 541 CCGAGCTGGA ATACGACTCA GAGCACAGCG ACTGGCACGG CTTTGACTGC CCAACTTTCT 601 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 661 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 721 GTGGAAAGGA CGATCCAACA CATGATGACA TGTCGAAACG CGTTAAGTCg acaatcaacc 781 tctggattac aaaatttgtg aaagatt