Transcript: Human NM_004491.5

Homo sapiens Rho GTPase activating protein 35 (ARHGAP35), mRNA.

Source:
NCBI, updated 2019-09-27
Taxon:
Homo sapiens (human)
Gene:
ARHGAP35 (2909)
Length:
9290
CDS:
402..4901

Additional Resources:

NCBI RefSeq record:
NM_004491.5
NBCI Gene record:
ARHGAP35 (2909)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_004491.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000276088 CATAATCGAGGCTACTCATAT pLKO_005 3239 CDS 100% 13.200 18.480 N ARHGAP35 n/a
2 TRCN0000022187 CGGTACATTAGAGATGCACAT pLKO.1 1023 CDS 100% 4.050 5.670 N ARHGAP35 n/a
3 TRCN0000276086 CGGTACATTAGAGATGCACAT pLKO_005 1023 CDS 100% 4.050 5.670 N ARHGAP35 n/a
4 TRCN0000022186 CGGGATAATCATTTAGTCCAT pLKO.1 2466 CDS 100% 2.640 3.696 N ARHGAP35 n/a
5 TRCN0000276087 TGAGCTGGCTTGGACCCATTT pLKO_005 5103 3UTR 100% 10.800 7.560 N ARHGAP35 n/a
6 TRCN0000022188 CGGTTGGTTCATGGGTACATT pLKO.1 2949 CDS 100% 5.625 3.938 N ARHGAP35 n/a
7 TRCN0000276090 CGGTTGGTTCATGGGTACATT pLKO_005 2949 CDS 100% 5.625 3.938 N ARHGAP35 n/a
8 TRCN0000022185 CCAGACAATCATTGAACTCTT pLKO.1 4664 CDS 100% 4.950 3.465 N ARHGAP35 n/a
9 TRCN0000022184 GCCCTTATTCTGAAACACATT pLKO.1 2025 CDS 100% 4.950 3.465 N ARHGAP35 n/a
10 TRCN0000276089 GCCCTTATTCTGAAACACATT pLKO_005 2025 CDS 100% 4.950 3.465 N ARHGAP35 n/a
11 TRCN0000166364 CACACACACACACACACACAA pLKO.1 8144 3UTR 100% 4.950 2.475 Y KAAG1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_004491.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

No results found.