Transcript: Human NM_005681.4

Homo sapiens TATA-box binding protein associated factor, RNA polymerase I subunit A (TAF1A), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-08-03
Taxon:
Homo sapiens (human)
Gene:
TAF1A (9015)
Length:
1910
CDS:
210..1562

Additional Resources:

NCBI RefSeq record:
NM_005681.4
NBCI Gene record:
TAF1A (9015)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_005681.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000255938 AGATTGTACCATCTCATAAAT pLKO_005 1114 CDS 100% 15.000 21.000 N TAF1A n/a
2 TRCN0000013334 CCGGATGCACTAAGAATATAA pLKO.1 1228 CDS 100% 15.000 21.000 N TAF1A n/a
3 TRCN0000013336 GCTCGGAAGTGAAATTCTATT pLKO.1 512 CDS 100% 13.200 18.480 N TAF1A n/a
4 TRCN0000013335 CGGGAAATATTAATCAACCTT pLKO.1 723 CDS 100% 3.000 4.200 N TAF1A n/a
5 TRCN0000255933 CAAGAGGTACTCACCAATTAT pLKO_005 978 CDS 100% 15.000 12.000 N TAF1A n/a
6 TRCN0000255936 GTAGATATTTCCGGTATATTT pLKO_005 1453 CDS 100% 15.000 12.000 N TAF1A n/a
7 TRCN0000255939 TATTGGCGTCATGAATTATTT pLKO_005 590 CDS 100% 15.000 12.000 N TAF1A n/a
8 TRCN0000255934 CTCCTTACAACATGCATTATA pLKO_005 617 CDS 100% 15.000 10.500 N TAF1A n/a
9 TRCN0000255931 CTGCATCATGGAATGCTTAAA pLKO_005 642 CDS 100% 13.200 9.240 N TAF1A n/a
10 TRCN0000265714 TAGCTAGAGAAACAATATTAC pLKO_005 1709 3UTR 100% 13.200 9.240 N TAF1A n/a
11 TRCN0000255935 TGATAAGTGTGCTTAAGATTT pLKO_005 1087 CDS 100% 13.200 9.240 N TAF1A n/a
12 TRCN0000255932 CCTTTGCTCTTTGTAACTATG pLKO_005 1732 3UTR 100% 10.800 7.560 N TAF1A n/a
13 TRCN0000013337 GCCAGGCTTTCATTTCAGCTA pLKO.1 1346 CDS 100% 2.640 1.848 N TAF1A n/a
14 TRCN0000255937 ATCAAATCCAAATGCCCATAT pLKO_005 1019 CDS 100% 10.800 6.480 N TAF1A n/a
15 TRCN0000013333 GCTTGGTAGATAGTTATTGAA pLKO.1 1607 3UTR 100% 5.625 3.375 N TAF1A n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_005681.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07338 pDONR223 100% 99.9% 100% None 1143C>G n/a
2 ccsbBroad304_07338 pLX_304 0% 99.9% 100% V5 1143C>G n/a
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