Construct: ORF ccsbBroad304_07338
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006309.1_s304c1, BRDN0000400097
- Derived from:
- ccsbBroadEn_07338
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TAF1A (9015)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07338
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9015 | TAF1A | TATA-box binding protein as... | NM_001201536.1 | 99.9% | 100% | 1143C>G |
| 2 | human | 9015 | TAF1A | TATA-box binding protein as... | NM_005681.4 | 99.9% | 100% | 1143C>G |
| 3 | human | 9015 | TAF1A | TATA-box binding protein as... | XM_006711612.1 | 91.4% | 91.5% | 289_290ins114;1029C>G |
| 4 | human | 9015 | TAF1A | TATA-box binding protein as... | XM_017002758.1 | 91.4% | 91.5% | 289_290ins114;1029C>G |
| 5 | human | 9015 | TAF1A | TATA-box binding protein as... | NM_139352.2 | 74.5% | 74.6% | 0_1ins342;801C>G |
| 6 | human | 9015 | TAF1A | TATA-box binding protein as... | XM_006711613.3 | 74.5% | 74.6% | 0_1ins342;801C>G |
| 7 | human | 9015 | TAF1A | TATA-box binding protein as... | XM_017002759.1 | 74.5% | 74.6% | 0_1ins342;801C>G |
| 8 | human | 9015 | TAF1A | TATA-box binding protein as... | XM_017002760.1 | 74.5% | 74.6% | 0_1ins342;801C>G |
| 9 | human | 9015 | TAF1A | TATA-box binding protein as... | XR_001737526.2 | 70.1% | (many diffs) | |
| 10 | human | 9015 | TAF1A | TATA-box binding protein as... | XM_005273343.4 | 67.6% | 67.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1416
- ORF length:
- 1350
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgag tgatttcagt gaagaattaa aagggcctgt gacagatgat gaagaagtgg 121 aaacatctgt gctcagtggt gcaggaatgc attttccttg gcttcaaaca tacgtagaaa 181 ctgtggccat tggagggaaa aggaggaagg attttgctca gacaacaagt gcttgtttaa 241 gttttatcca agaagctctg ctgaagcacc aatggcagca agctgcagaa tacatgtaca 301 gttattttca gaccttggaa gattcagata gctacaaaag gcaggctgca cctgagatta 361 tttggaagct cggaagtgaa attctatttt atcatcccaa aagcaacatg gagagtttca 421 atacttttgc taaccggatg aaaaatattg gcgtcatgaa ttatttaaag atctccttac 481 aacatgcatt ataccttctg catcatggaa tgcttaaaga tgctaagaga aatctgagtg 541 aggcagagac atggagacat ggtgaaaata cgtcttcccg ggaaatatta atcaacctta 601 ttcaggccta taaagggctt ttacagtatt atacctggtc tgaaaagaag atggaattgt 661 caaagcttga taaggatgat tatgcttaca atgcagtagc ccaggatgtg ttcaaccaca 721 gctggaagac atctgcaaat atttctgcat tgattaaaat tcctggagtt tgggaccctt 781 ttgtgaagag ttatgtagaa atgctggaat tctatgggga tcgagatgga gcccaagagg 841 tactcaccaa ttatgcatat gatgaaaagt ttccatcaaa tccaaatgcc catatctact 901 tatacaactt tctaaagaga cagaaggcac caagatcaaa attgataagt gtgcttaaga 961 ttttgtatca gattgtacca tctcataaat tgatgttgga attccataca ttacttagaa 1021 aatcagaaaa agaagaacac cgtaaactgg ggttggaggt attatttgga gtcttagatt 1081 ttgccggatg cactaagaat ataactgctt ggaaatactt ggcaaaatat ctgaaaaata 1141 tcttaatggg aaaccacctt gcgtgggttc aagaagagtg gaactccagg aaaaactggt 1201 ggccagggtt tcatttcagc tacttttggg caaaaagtga ttggaaggaa gatacagctt 1261 tggcctgtga gaaagctttt gtggctggtt tactgttagg aaaaggttgt agatatttcc 1321 ggtatatttt aaagcaagat caccaaatct tagggaagaa aattaagcgg atgaagagat 1381 ctgtgaaaaa atacagtatt gtaaatccaa gactctgccc aactttcttg tacaaagtgg 1441 ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg 1501 ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta