Transcript: Human NM_005732.4

Homo sapiens RAD50 double strand break repair protein (RAD50), mRNA.

Source:
NCBI, updated 2019-09-30
Taxon:
Homo sapiens (human)
Gene:
RAD50 (10111)
Length:
8272
CDS:
349..4287

Additional Resources:

NCBI RefSeq record:
NM_005732.4
NBCI Gene record:
RAD50 (10111)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_005732.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000040104 CGCCTAAAGAACGACATAGAA pLKO.1 2635 CDS 100% 5.625 7.875 N RAD50 n/a
2 TRCN0000286222 CGCCTAAAGAACGACATAGAA pLKO_005 2635 CDS 100% 5.625 7.875 N RAD50 n/a
3 TRCN0000293672 ATCCGAGCTAGAGATTCATTA pLKO_005 1438 CDS 100% 13.200 10.560 N RAD50 n/a
4 TRCN0000009838 GAGATTCGTGATCAGATTACA pLKO.1 1012 CDS 100% 5.625 4.500 N RAD50 n/a
5 TRCN0000040105 CGACCATCATTGAATGTCTAA pLKO.1 476 CDS 100% 4.950 3.960 N RAD50 n/a
6 TRCN0000040103 CGGGTCTTGATTCAGTCACAT pLKO.1 5372 3UTR 100% 4.950 3.960 N RAD50 n/a
7 TRCN0000298547 GAACAAGGATCTGGATATTTA pLKO_005 3699 CDS 100% 15.000 10.500 N RAD50 n/a
8 TRCN0000293736 CTCATATCAACTTAGTCAATA pLKO_005 4355 3UTR 100% 13.200 9.240 N RAD50 n/a
9 TRCN0000293673 TCCATTGAAGAATCGTCTAAA pLKO_005 1095 CDS 100% 13.200 9.240 N RAD50 n/a
10 TRCN0000010354 CATCTATCTGTGCTAGGTTAA pLKO.1 5492 3UTR 100% 10.800 7.560 N RAD50 n/a
11 TRCN0000040106 GCCAAATTGAACAAGGAACTA pLKO.1 2131 CDS 100% 4.950 3.465 N RAD50 n/a
12 TRCN0000040107 GCTGTGCTAAATAATGTCATT pLKO.1 793 CDS 100% 4.950 3.465 N RAD50 n/a
13 TRCN0000009839 TACCTATCGTGGACAAGATAT pLKO.1 3807 CDS 100% 0.000 0.000 N RAD50 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_005732.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

No results found.