Transcript: Mouse NM_019459.2

Mus musculus nephrosis 1, nephrin (Nphs1), mRNA.

Source:
NCBI, updated 2017-06-11
Taxon:
Mus musculus (mouse)
Gene:
Nphs1 (54631)
Length:
5661
CDS:
87..3857

Additional Resources:

NCBI RefSeq record:
NM_019459.2
NBCI Gene record:
Nphs1 (54631)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_019459.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000094341 CACTCCCATAAAGGCTTCGTT pLKO.1 803 CDS 100% 3.000 2.400 N Nphs1 n/a
2 TRCN0000094343 ACACTGGAAGAGGTGTCATAT pLKO.1 3582 CDS 100% 13.200 9.240 N Nphs1 n/a
3 TRCN0000094342 CAAGCCTTCACAGGTATTGAA pLKO.1 3606 CDS 100% 5.625 3.938 N Nphs1 n/a
4 TRCN0000094339 CCCTCCAGTTAACTTGTCTTT pLKO.1 1847 CDS 100% 4.950 3.465 N Nphs1 n/a
5 TRCN0000094340 CGTGTTATACCCTCCAGAGTT pLKO.1 2051 CDS 100% 4.950 3.465 N Nphs1 n/a
6 TRCN0000182716 CCTGAGTTCAATTCCCAGCAA pLKO.1 4157 3UTR 100% 2.640 1.320 Y BC028528 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_019459.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

No results found.