Transcript: Human NM_031244.3

Homo sapiens sirtuin 5 (SIRT5), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-09-10
Taxon:
Homo sapiens (human)
Gene:
SIRT5 (23408)
Length:
2426
CDS:
291..1190

Additional Resources:

NCBI RefSeq record:
NM_031244.3
NBCI Gene record:
SIRT5 (23408)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_031244.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000232660 GAGATCCATGGTAGCTTATTT pLKO_005 756 CDS 100% 15.000 21.000 N SIRT5 n/a
2 TRCN0000257335 GCGTGCCAGTGGCTGAATTTA pLKO_005 1093 CDS 100% 15.000 21.000 N SIRT5 n/a
3 TRCN0000018546 CGTCCACACGAAACCAGATTT pLKO.1 358 CDS 100% 13.200 18.480 N SIRT5 n/a
4 TRCN0000018543 CCAGCTATATTGTGGCCTGAA pLKO.1 329 CDS 100% 4.050 5.670 N SIRT5 n/a
5 TRCN0000018547 GAGCTGGTGTTAGTGCAGAAA pLKO.1 463 CDS 100% 4.950 3.960 N SIRT5 n/a
6 TRCN0000232659 GATTGATTTCCCAGCTATATT pLKO_005 319 CDS 100% 15.000 10.500 N SIRT5 n/a
7 TRCN0000232661 AGAATTACAAGAGTCCAATTT pLKO_005 811 CDS 100% 13.200 9.240 N SIRT5 n/a
8 TRCN0000018544 GAGTCCAATTTGTCCAGCTTT pLKO.1 821 CDS 100% 4.950 3.465 N SIRT5 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_031244.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07880 pDONR223 98.4% 94.6% 89.6% None (many diffs) n/a
2 ccsbBroad304_07880 pLX_304 0% 94.6% 89.6% V5 (not translated due to prior stop codon) (many diffs) n/a
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