Construct: ORF ccsbBroad304_07880
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008966.1_s304c1, BRDN0000405043
- Derived from:
- ccsbBroadEn_07880
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- SIRT5 (23408)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_07880
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23408 | SIRT5 | sirtuin 5 | NM_012241.5 | 99.8% | 100% | 657C>T |
| 2 | human | 23408 | SIRT5 | sirtuin 5 | XM_005248968.4 | 99.8% | 100% | 657C>T |
| 3 | human | 23408 | SIRT5 | sirtuin 5 | XM_024446386.1 | 99.8% | 100% | 657C>T |
| 4 | human | 23408 | SIRT5 | sirtuin 5 | NM_031244.3 | 94.6% | 89.6% | (many diffs) |
| 5 | human | 23408 | SIRT5 | sirtuin 5 | NM_001193267.2 | 94% | 94.1% | 562_563ins54;603C>T |
| 6 | human | 23408 | SIRT5 | sirtuin 5 | XM_017010620.2 | 94% | 91.7% | (many diffs) |
| 7 | human | 23408 | SIRT5 | sirtuin 5 | XM_011514444.2 | 87.3% | 87.4% | 0_1ins117;540C>T |
| 8 | human | 23408 | SIRT5 | sirtuin 5 | XM_017010622.2 | 87.3% | 87.4% | 0_1ins117;540C>T |
| 9 | human | 23408 | SIRT5 | sirtuin 5 | XM_017010623.1 | 87.3% | 87.4% | 0_1ins117;540C>T |
| 10 | human | 23408 | SIRT5 | sirtuin 5 | XM_017010621.1 | 82.8% | 58.7% | 616_617ins124;807_849del |
| 11 | human | 23408 | SIRT5 | sirtuin 5 | XM_024446387.1 | 82.8% | 58.7% | 616_617ins124;807_849del |
| 12 | human | 23408 | SIRT5 | sirtuin 5 | XM_017010624.2 | 81.1% | 65.6% | (many diffs) |
| 13 | human | 23408 | SIRT5 | sirtuin 5 | XM_024446388.1 | 79.3% | 67.2% | 616_617ins124;733_734insGTT;738_739ins65 |
| 14 | human | 23408 | SIRT5 | sirtuin 5 | NM_001242827.1 | 65% | 65.1% | 0_1ins324;333C>T |
| 15 | mouse | 68346 | Sirt5 | sirtuin 5 | NM_178848.3 | 83.9% | 86.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 996
- ORF length:
- 930
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttgtgatgcg acctctccag attgtcccaa gtcgattgat ttcccagcta tattgtggcc 121 tgaagcctcc agcgtccaca cgaaaccaga tttgcctgaa aatggctcgg ccaagttcaa 181 gtatggcaga ttttcgaaag ttttttgcaa aagcaaagca catagtcatc atctcaggag 241 ctggtgttag tgcagaaagt ggtgttccga ccttcagagg agctggaggt tattggagaa 301 aatggcaagc ccaggacctg gcgactcccc tggcctttgc ccacaacccg tcccgggtgt 361 gggagttcta ccactaccgg cgggaggtca tggggagcaa ggagcccaac gccgggcacc 421 gcgccatagc cgagtgtgag acccggctgg gcaagcaggg ccggcgagtc gtggtcatca 481 cccagaacat cgatgagctg caccgcaagg ctggcaccaa gaaccttctg gagatccatg 541 gtagcttatt taaaactcga tgtacctctt gtggagttgt ggctgagaat tacaagagtc 601 caatttgtcc agctttatca ggaaaaggtg ctccagaacc tggaactcaa gatgccagca 661 tcccagttga gaaacttccc cggtgtgaag aggcaggctg cgggggcttg ctgcgacctc 721 atgtcgtgtg gtttggagaa aacctggatc ctgccattct ggaggaggtt gacagagagc 781 tcgcccactg tgatttatgt ctagtggtgg gcacttcctc tgtggtgtac ccagcagcca 841 tgtttgcccc ccaggtggct gccaggggcg tgccagtggc tgaatttaac acggagacca 901 ccccagctac gaacagattc aggtttcatt tccagggacc ctgtggaacg actcttcctg 961 aagcccttgc ctgtcatgaa aatgaaactg tttcttaacc aactttcttg tacaaagtgg 1021 ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg 1081 ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta