Construct: ORF ccsbBroad304_07880

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008966.1_s304c1, BRDN0000405043
Derived from:
ccsbBroadEn_07880
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
SIRT5 (23408)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_07880
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23408 SIRT5 sirtuin 5 NM_012241.5 99.8% 100% 657C>T
2 human 23408 SIRT5 sirtuin 5 XM_005248968.4 99.8% 100% 657C>T
3 human 23408 SIRT5 sirtuin 5 XM_024446386.1 99.8% 100% 657C>T
4 human 23408 SIRT5 sirtuin 5 NM_031244.3 94.6% 89.6% (many diffs)
5 human 23408 SIRT5 sirtuin 5 NM_001193267.2 94% 94.1% 562_563ins54;603C>T
6 human 23408 SIRT5 sirtuin 5 XM_017010620.2 94% 91.7% (many diffs)
7 human 23408 SIRT5 sirtuin 5 XM_011514444.2 87.3% 87.4% 0_1ins117;540C>T
8 human 23408 SIRT5 sirtuin 5 XM_017010622.2 87.3% 87.4% 0_1ins117;540C>T
9 human 23408 SIRT5 sirtuin 5 XM_017010623.1 87.3% 87.4% 0_1ins117;540C>T
10 human 23408 SIRT5 sirtuin 5 XM_017010621.1 82.8% 58.7% 616_617ins124;807_849del
11 human 23408 SIRT5 sirtuin 5 XM_024446387.1 82.8% 58.7% 616_617ins124;807_849del
12 human 23408 SIRT5 sirtuin 5 XM_017010624.2 81.1% 65.6% (many diffs)
13 human 23408 SIRT5 sirtuin 5 XM_024446388.1 79.3% 67.2% 616_617ins124;733_734insGTT;738_739ins65
14 human 23408 SIRT5 sirtuin 5 NM_001242827.1 65% 65.1% 0_1ins324;333C>T
15 mouse 68346 Sirt5 sirtuin 5 NM_178848.3 83.9% 86.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
996
ORF length:
930
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttgtgatgcg acctctccag attgtcccaa gtcgattgat ttcccagcta tattgtggcc
121tgaagcctcc agcgtccaca cgaaaccaga tttgcctgaa aatggctcgg ccaagttcaa
181gtatggcaga ttttcgaaag ttttttgcaa aagcaaagca catagtcatc atctcaggag
241ctggtgttag tgcagaaagt ggtgttccga ccttcagagg agctggaggt tattggagaa
301aatggcaagc ccaggacctg gcgactcccc tggcctttgc ccacaacccg tcccgggtgt
361gggagttcta ccactaccgg cgggaggtca tggggagcaa ggagcccaac gccgggcacc
421gcgccatagc cgagtgtgag acccggctgg gcaagcaggg ccggcgagtc gtggtcatca
481cccagaacat cgatgagctg caccgcaagg ctggcaccaa gaaccttctg gagatccatg
541gtagcttatt taaaactcga tgtacctctt gtggagttgt ggctgagaat tacaagagtc
601caatttgtcc agctttatca ggaaaaggtg ctccagaacc tggaactcaa gatgccagca
661tcccagttga gaaacttccc cggtgtgaag aggcaggctg cgggggcttg ctgcgacctc
721atgtcgtgtg gtttggagaa aacctggatc ctgccattct ggaggaggtt gacagagagc
781tcgcccactg tgatttatgt ctagtggtgg gcacttcctc tgtggtgtac ccagcagcca
841tgtttgcccc ccaggtggct gccaggggcg tgccagtggc tgaatttaac acggagacca
901ccccagctac gaacagattc aggtttcatt tccagggacc ctgtggaacg actcttcctg
961aagcccttgc ctgtcatgaa aatgaaactg tttcttaacc aactttcttg tacaaagtgg
1021ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg
1081ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta

Download FASTA (ORF) (Full)