Transcript: Human NM_033266.4

Homo sapiens endoplasmic reticulum to nucleus signaling 2 (ERN2), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-09-27
Taxon:
Homo sapiens (human)
Gene:
ERN2 (10595)
Length:
3337
CDS:
36..2816

Additional Resources:

NCBI RefSeq record:
NM_033266.4
NBCI Gene record:
ERN2 (10595)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001162277 GAGACCACTTCTTACCACCA pXPR_003 GGG 931 33% 9 1.0249 ERN2 ERN2 75848
2 BRDN0001145316 GTGGAGACTTCCATCCAAGG pXPR_003 TGG 136 5% 2 0.7738 ERN2 ERN2 75845
3 BRDN0001147211 TTGGGGACCCAAAAACAACA pXPR_003 GGG 293 11% 4 0.268 ERN2 ERN2 75846
4 BRDN0001162553 CTGAGCAACTCACCGTAGTG pXPR_003 GGG 1542 55% 14 -0.1261 ERN2 ERN2 75847
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_033266.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000195361 CGTCATCGAAGGACCAATGTA pLKO.1 239 CDS 100% 5.625 7.875 N ERN2 n/a
2 TRCN0000021430 GCGTGTTCTACTACGTGCTTT pLKO.1 2167 CDS 100% 4.950 6.930 N ERN2 n/a
3 TRCN0000021432 ACTGGCTTCTATGTCTCTAAA pLKO.1 918 CDS 100% 13.200 9.240 N ERN2 n/a
4 TRCN0000195136 CTGGCTTCTATGTCTCTAAAG pLKO.1 919 CDS 100% 10.800 7.560 N ERN2 n/a
5 TRCN0000021431 CCACCTGCACTCTTTACACAT pLKO.1 1913 CDS 100% 4.950 3.465 N ERN2 n/a
6 TRCN0000021433 CTGAGAAAGTTCCGGTCCTAT pLKO.1 2538 CDS 100% 4.950 3.465 N ERN2 n/a
7 TRCN0000195546 CTGCAGACTTTGCTCACATCT pLKO.1 1441 CDS 100% 4.950 3.465 N ERN2 n/a
8 TRCN0000179143 GAAGGATGAAACTGGCTTCTA pLKO.1 908 CDS 100% 4.950 3.465 N ERN2 n/a
9 TRCN0000147669 GAAGATTTCCTTCAATCCCAA pLKO.1 1583 CDS 100% 2.640 1.848 N ERN2 n/a
10 TRCN0000199493 GCTGGCTCACTGAAGAGCTGA pLKO.1 2849 3UTR 100% 0.880 0.616 N ERN2 n/a
11 TRCN0000021429 CACACCTGTAATCCCAGCATT pLKO.1 2910 3UTR 100% 4.950 2.475 Y ERN2 n/a
12 TRCN0000138998 CACACCTGTAATCCCAGCATT pLKO.1 2910 3UTR 100% 4.950 2.475 Y P3H4 n/a
13 TRCN0000344020 CACACCTGTAATCCCAGCATT pLKO_005 2910 3UTR 100% 4.950 2.475 Y P3H4 n/a
14 TRCN0000180418 GATCACTTGAGCTCAGGAGTT pLKO.1 2950 3UTR 100% 4.050 2.025 Y ERN2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_033266.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

No results found.