Transcript: Human NM_033642.3

Homo sapiens fibroblast growth factor 13 (FGF13), transcript variant 6, mRNA.

Source:
NCBI, updated 2019-09-29
Taxon:
Homo sapiens (human)
Gene:
FGF13 (2258)
Length:
18786
CDS:
85..663

Additional Resources:

NCBI RefSeq record:
NM_033642.3
NBCI Gene record:
FGF13 (2258)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_033642.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000430160 TAGATTGAGCTTGTGCATAAG pLKO_005 846 3UTR 100% 10.800 15.120 N FGF13 n/a
2 TRCN0000059151 GAGCCACAATGAATCAACGTA pLKO.1 642 CDS 100% 3.000 2.400 N FGF13 n/a
3 TRCN0000059148 CAGCACTTACACTCTGTTTAA pLKO.1 213 CDS 100% 13.200 9.240 N FGF13 n/a
4 TRCN0000423466 GAACAAGCCTGCAGCTCATTT pLKO_005 486 CDS 100% 13.200 9.240 N FGF13 n/a
5 TRCN0000066936 CCTCAGCTTAAGGGTATAGTT pLKO.1 115 CDS 100% 5.625 3.938 N Fgf13 n/a
6 TRCN0000059149 AGGGTATAGTTACCAAGCTAT pLKO.1 125 CDS 100% 4.950 3.465 N FGF13 n/a
7 TRCN0000066934 GACAGCACTTACACTCTGTTT pLKO.1 211 CDS 100% 4.950 3.465 N Fgf13 n/a
8 TRCN0000059152 GAGATCATGAAAGGCAACCAT pLKO.1 457 CDS 100% 3.000 2.100 N FGF13 n/a
9 TRCN0000059150 CAAGCTGTACTTGGCAATGAA pLKO.1 282 CDS 100% 5.625 3.375 N FGF13 n/a
10 TRCN0000166463 CCTGTGTCCATGTGTTCTCAT pLKO.1 13229 3UTR 100% 4.950 2.475 Y SPC25 n/a
11 TRCN0000009295 GCAGGTTTGTTACATAGGTAA pLKO.1 13069 3UTR 100% 4.950 2.475 Y OR11A1 n/a
12 TRCN0000149064 GCAGGTTTGTTACATAGGTAT pLKO.1 14059 3UTR 100% 4.950 2.475 Y GLIPR1L2 n/a
13 TRCN0000135406 GCCTAGATTTCAGAGGATGTA pLKO.1 16743 3UTR 100% 4.950 2.475 Y FLJ44955 n/a
14 TRCN0000166364 CACACACACACACACACACAA pLKO.1 5862 3UTR 100% 4.950 2.475 Y KAAG1 n/a
15 TRCN0000178741 CACACACATACACACACACAA pLKO.1 3464 3UTR 100% 4.950 2.475 Y Cstad n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_033642.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00559 pDONR223 100% 76.6% 75.1% None (many diffs) n/a
2 ccsbBroad304_00559 pLX_304 0% 76.6% 75.1% V5 (many diffs) n/a
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