Transcript: Mouse NM_138681.4

Mus musculus breast carcinoma amplified sequence 3 (Bcas3), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-05-27
Taxon:
Mus musculus (mouse)
Gene:
Bcas3 (192197)
Length:
3692
CDS:
162..2948

Additional Resources:

NCBI RefSeq record:
NM_138681.4
NBCI Gene record:
Bcas3 (192197)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_138681.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000251992 ACGGATGATCTAGATCTTAAC pLKO_005 2505 CDS 100% 10.800 15.120 N Bcas3 n/a
2 TRCN0000251995 TGTCACCACGAGTAGTCAATC pLKO_005 1501 CDS 100% 10.800 15.120 N Bcas3 n/a
3 TRCN0000251994 TCAGTACTGCTCCCAAGATAA pLKO_005 2056 CDS 100% 13.200 9.240 N Bcas3 n/a
4 TRCN0000251993 AGTGACGCCTCTTGCGCAAAT pLKO_005 1736 CDS 100% 10.800 7.560 N Bcas3 n/a
5 TRCN0000257714 CCACCTTCCCTAAGCTTAGTG pLKO_005 3094 3UTR 100% 4.950 3.465 N Bcas3 n/a
6 TRCN0000130230 CTTGGTGTTTGTAAGAGCATT pLKO.1 606 CDS 100% 4.950 3.465 N BCAS3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_138681.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12092 pDONR223 100% 37.1% 40.1% None (many diffs) n/a
2 ccsbBroad304_12092 pLX_304 0% 37.1% 40.1% V5 (many diffs) n/a
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