Construct: ORF ccsbBroadEn_12092

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010378.1_s300c1, BRDN0000388401
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
BCAS3 (54828)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 54828 BCAS3 BCAS3 microtubule associate... XM_024450813.1 57% 57% 1_882del
2 human 54828 BCAS3 BCAS3 microtubule associate... XM_017024796.1 55.7% 55.7% 1_882del;950_994del
3 human 54828 BCAS3 BCAS3 microtubule associate... NM_017679.5 42.7% 42.7% 1_1569del
4 human 54828 BCAS3 BCAS3 microtubule associate... NM_001099432.3 42% 42% 1_1569del;1637_1681del
5 human 54828 BCAS3 BCAS3 microtubule associate... NM_001330414.2 41.7% 41.7% 1_1569del;2593_2658del
6 human 54828 BCAS3 BCAS3 microtubule associate... NM_001353145.2 41.3% 41.3% 1_1659del
7 human 54828 BCAS3 BCAS3 microtubule associate... NM_001330413.2 41% 41% 1_1569del;1637_1681del;2638_2703del
8 human 54828 BCAS3 BCAS3 microtubule associate... NM_001353144.2 40.7% 40.7% 1_1659del;1727_1771del
9 human 54828 BCAS3 BCAS3 microtubule associate... XM_011524943.3 40.4% 40.4% 1_1659del;2683_2748del
10 human 54828 BCAS3 BCAS3 microtubule associate... XM_011524942.3 39.7% 39.7% 1_1659del;1727_1771del;2728_2793del
11 human 54828 BCAS3 BCAS3 microtubule associate... XM_017024788.2 38.8% 38.7% 1_1659del;1727_1771del;2561_2701del
12 human 54828 BCAS3 BCAS3 microtubule associate... NM_001320470.3 38.7% 35.6% (many diffs)
13 human 54828 BCAS3 BCAS3 microtubule associate... XM_017024790.2 38.1% 35.1% (many diffs)
14 human 54828 BCAS3 BCAS3 microtubule associate... XM_017024787.2 37.9% 37.8% (many diffs)
15 human 54828 BCAS3 BCAS3 microtubule associate... XM_011524941.2 37.9% 37.9% 1_1569del;2424_2702del;2872_2937del
16 human 54828 BCAS3 BCAS3 microtubule associate... XM_017024786.2 37.3% 37.3% (many diffs)
17 human 54828 BCAS3 BCAS3 microtubule associate... XM_017024793.2 37.3% 37.3% 1_1569del;2592_2682del;2718_2719ins112
18 human 54828 BCAS3 BCAS3 microtubule associate... XM_017024784.2 37.1% 37.1% 1_1659del;1727_1771del;2559_2837del
19 human 54828 BCAS3 BCAS3 microtubule associate... XM_011524940.2 36.8% 36.8% 1_1659del;2514_2792del;2962_3027del
20 human 54828 BCAS3 BCAS3 microtubule associate... NM_001353146.2 36.8% 36.7% (many diffs)
21 human 54828 BCAS3 BCAS3 microtubule associate... XM_011524939.2 36.3% 36.3% (many diffs)
22 human 54828 BCAS3 BCAS3 microtubule associate... XM_017024789.2 35.6% 35.6% (many diffs)
23 human 54828 BCAS3 BCAS3 microtubule associate... XM_017024783.2 33.8% 31.1% (many diffs)
24 human 54828 BCAS3 BCAS3 microtubule associate... XM_017024785.2 32.6% 32.5% (many diffs)
25 human 54828 BCAS3 BCAS3 microtubule associate... XM_024450812.1 31.7% 31.3% (many diffs)
26 human 54828 BCAS3 BCAS3 microtubule associate... XM_017024795.2 31.3% 30.9% (many diffs)
27 human 54828 BCAS3 BCAS3 microtubule associate... XM_011524946.3 30.3% 29.9% (many diffs)
28 human 54828 BCAS3 BCAS3 microtubule associate... XM_011524947.2 30.3% 30.1% (many diffs)
29 human 54828 BCAS3 BCAS3 microtubule associate... XM_011524948.3 30.2% 29.8% (many diffs)
30 human 54828 BCAS3 BCAS3 microtubule associate... XM_011524949.3 30.1% 30% (many diffs)
31 human 54828 BCAS3 BCAS3 microtubule associate... XM_011524950.2 30% 29.8% (many diffs)
32 human 54828 BCAS3 BCAS3 microtubule associate... XM_011524951.3 29.9% 29.8% (many diffs)
33 human 54828 BCAS3 BCAS3 microtubule associate... XM_017024794.2 29.9% 29.7% (many diffs)
34 human 54828 BCAS3 BCAS3 microtubule associate... XR_001752547.2 20.9% (many diffs)
35 human 54828 BCAS3 BCAS3 microtubule associate... XR_934493.3 17.6% (many diffs)
36 mouse 192197 Bcas3 breast carcinoma amplified ... NM_001166642.1 37.8% 40.8% (many diffs)
37 mouse 192197 Bcas3 breast carcinoma amplified ... NM_001166643.1 37.8% 40.8% (many diffs)
38 mouse 192197 Bcas3 breast carcinoma amplified ... XM_006532541.2 37.8% 40.8% (many diffs)
39 mouse 192197 Bcas3 breast carcinoma amplified ... XM_017314343.1 37.2% 40.2% (many diffs)
40 mouse 192197 Bcas3 breast carcinoma amplified ... NM_138681.4 37.1% 40.1% (many diffs)
41 mouse 192197 Bcas3 breast carcinoma amplified ... XM_006532540.2 37.1% 40.1% (many diffs)
42 mouse 192197 Bcas3 breast carcinoma amplified ... XM_006532538.2 36.6% 39.6% (many diffs)
43 mouse 192197 Bcas3 breast carcinoma amplified ... XM_006532536.3 35.8% 38.7% (many diffs)
44 mouse 192197 Bcas3 breast carcinoma amplified ... XM_017314342.1 34.3% 35% (many diffs)
45 mouse 192197 Bcas3 breast carcinoma amplified ... XM_006532537.3 33.3% 34% (many diffs)
46 mouse 192197 Bcas3 breast carcinoma amplified ... XM_006532539.3 32% 34.4% (many diffs)
47 mouse 192197 Bcas3 breast carcinoma amplified ... XM_011248810.2 27.5% 29% (many diffs)
48 mouse 192197 Bcas3 breast carcinoma amplified ... XM_006532542.3 26.3% 28.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1236
ORF length:
1170
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACATAAAAG
61TTGTCATGGT AGTGATGCCT CTTGCACAAA TCAAGCAGCC AATGACATTG GGGACCATCA
121CCAAACGAAC CGGCAAAGTT AAACCTCCTC CACAAATTTC ACCCAGCAAA TCGATGGGCG
181GAGAATTTTG TGTGGCTGCT ATCTTCGGAA CATCCAGGTC ATGGTTTGCA AATAATGCAG
241GTCTGAAAAG AGAAAAAGAT CAGTCCAAAC AAGTTGTAGT TGAGTCCCTG TACATTATCA
301GTTGCTATGG CACCTTAGTG GAACACATGA TGGAGCCGCG ACCCCTCAGC ACTGCACCCA
361AGATTAGTGA CGACACACCA CTGGAAATGA TGACATCGCC TCGAGCCAGC TGGACTCTGG
421TTAGAACCCC TCAATGGAAT GAATTGCAGC CACCGTTTAA TGCAAACCAC CCTCTGCTCC
481TCGCTGCAGA TGCAGTACAG TATTATCAGT TCCTGCTTGC TGGCCTGGTT CCCCCTGGAA
541GTCCTGGGCC CATTACTCGA CATGGGTCTT ACGACAGTTT AGCTTCTGAC CATAGTGGAC
601AGGAAGATGA AGAATGGCTT TCCCAGGTTG AAATTGTAAC ACACACTGGA CCCCATAGAC
661GTCTGTGGAT GGGTCCACAG TTCCAGTTCA AAACCATCCA TCCCTCAGGC CAAACCACAG
721TTATCTCATC CAGTTCATCT GTGTTGCAGT CTCATGGTCC GAGTGACACG CCACAGCCTC
781TTTTGGATTT TGATACAGAT GATCTTGATC TCAACAGTCT CAGGATCCAG CCAGTCCGCT
841CTGACCCCGT CAGCATGCCA GGGTCATCCC GTCCAGTCTC TGATCGAAGG GGAGTTTCCA
901CAGTGATTGA TGCTGCCTCA GGTACCTTTG ACAGGAGCGT GACCCTGCTG GAGGTGTGCG
961GGAGCTGGCC TGAGGGCTTC GGGCTGCGGC ACATGTCCTC CATGGAGCAC ACGGAGGAGG
1021GCCTCCGGGA GCGACTTGCC GACGCCATGG CCGAGTCACC TAGCCGGGAC GTCGTGGGAT
1081CCGGAACAGA ACTTCAGCGA GAGGGAAGCA TCGAGACTCT GAGTAACAGC TCAGGCTCCA
1141CCAGCGGCAG CATACCAAGA AACTTTGATG GCTACCGATC TCCGCTGCCC ACCAATGAGA
1201GCCAGCCCCT CAGCCTCTTC CCGACTGGCT TCCCGTACCC AACTTTCTTG TACAAAGTtg
1261gcattataag aaagcattgc ttatcaattt gttgcaacga ac

Download FASTA (ORF) (Full)