Transcript: Human NM_144635.5

Homo sapiens family with sequence similarity 131 member A (FAM131A), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-05-02
Taxon:
Homo sapiens (human)
Gene:
FAM131A (131408)
Length:
2437
CDS:
32..1132

Additional Resources:

NCBI RefSeq record:
NM_144635.5
NBCI Gene record:
FAM131A (131408)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_144635.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000263482 CACCTCTCCATCGTCTCTAAA pLKO_005 1689 3UTR 100% 13.200 9.240 N FAM131A n/a
2 TRCN0000263486 ATGAGCTGCTTCTCGCCAAAC pLKO_005 864 CDS 100% 6.000 4.200 N FAM131A n/a
3 TRCN0000263485 CCACTGATGACTCCTATGATG pLKO_005 609 CDS 100% 4.950 3.465 N FAM131A n/a
4 TRCN0000167861 GCTTTGGAATGATGAAAGCAT pLKO.1 1782 3UTR 100% 0.300 0.210 N FAM131A n/a
5 TRCN0000265011 AGGACTTGCCAGAGGTGAATG pLKO_005 249 CDS 100% 10.800 5.400 Y Fam131a n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_144635.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14379 pDONR223 100% 76.6% .8% None 1_255del;262delC n/a
2 ccsbBroad304_14379 pLX_304 0% 76.6% .8% V5 (not translated due to prior stop codon) 1_255del;262delC n/a
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