Construct: ORF ccsbBroad304_14379
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006455.1_s304c1, BRDN0000399820
- Derived from:
- ccsbBroadEn_14379
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- FAM131A (131408)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14379
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 131408 | FAM131A | family with sequence simila... | NM_001171093.1 | 99.8% | 1% | 7delC |
| 2 | human | 131408 | FAM131A | family with sequence simila... | NM_001366133.1 | 99.8% | 1% | 7delC |
| 3 | human | 131408 | FAM131A | family with sequence simila... | NM_001366134.1 | 99.8% | 1% | 7delC |
| 4 | human | 131408 | FAM131A | family with sequence simila... | XM_005247114.3 | 99.8% | 1% | 7delC |
| 5 | human | 131408 | FAM131A | family with sequence simila... | NM_144635.5 | 76.6% | .8% | 1_255del;262delC |
| 6 | mouse | 78408 | Fam131a | family with sequence simila... | XM_006522740.3 | 87.1% | 1% | (many diffs) |
| 7 | mouse | 78408 | Fam131a | family with sequence simila... | XM_006522741.3 | 87.1% | 1% | (many diffs) |
| 8 | mouse | 78408 | Fam131a | family with sequence simila... | XM_006522742.3 | 87.1% | 1% | (many diffs) |
| 9 | mouse | 78408 | Fam131a | family with sequence simila... | XM_006522738.3 | 68.6% | .8% | (many diffs) |
| 10 | mouse | 78408 | Fam131a | family with sequence simila... | NM_133778.2 | 66.9% | .8% | (many diffs) |
| 11 | mouse | 78408 | Fam131a | family with sequence simila... | XM_017317161.1 | 43.3% | 1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 90
- ORF length:
- 24
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgct gccaagtccc ggcgagccct aactatccag gagatcgctg cgctggccag 121 gtcctccctg catggtattt cccaggtggt gaaggaccac gtgaccaagc ctaccgccat 181 ggcccagggc cgagtggctc acctcattga gtggaagggc tggagcaagc cgagtgactc 241 acctgctgcc ctggaatcag ccttttcctc ctattcagac ctcagcgagg gcgaacaaga 301 ggctcgcttt gcagcaggag tggctgagca gtttgccatc gcggaagcca agctccgagc 361 atggtcttcg gtggatggcg aggactccac tgatgactcc tatgatgagg actttgctgg 421 gggaatggac acagacatgg ctgggcagct gcccctgggg ccgcacctcc aggacctgtt 481 caccggccac cggttctccc ggcctgtgcg ccagggctcc gtggagcctg agagcgactg 541 ctcacagacc gtgtccccag acaccctgtg ctctagtctg tgcagcctgg aggatgggtt 601 gttgggctcc ccggcccggc tggcctccca gctgctgggc gatgagctgc ttctcgccaa 661 actgcccccc agccgggaaa gtgccttccg cagcctgggc ccactggagg cccaggactc 721 actctacaac tcgcccctca cagagtcctg cctttccccc gcggaggagg agccagcccc 781 ctgcaaggac tgccagccac tctgcccacc actaacgggc agctgggaac ggcagcggca 841 agcctctgac ctggcctctt ctggggtggt gtccttagat gaggatgagg cagagccaga 901 ggaacagtgc ccaactttct tgtacaaagt ggttggtaag cctatcccta accctctcct 961 cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta agtcgacaat 1021 caacctctgg atta