Transcript: Human NM_153252.5

Homo sapiens bromodomain and WD repeat domain containing 3 (BRWD3), mRNA.

Source:
NCBI, updated 2019-08-22
Taxon:
Homo sapiens (human)
Gene:
BRWD3 (254065)
Length:
12921
CDS:
407..5815

Additional Resources:

NCBI RefSeq record:
NM_153252.5
NBCI Gene record:
BRWD3 (254065)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_153252.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000378280 GTAGTCGCTTTGGTCATATTT pLKO_005 870 CDS 100% 15.000 21.000 N BRWD3 n/a
2 TRCN0000226212 TGATAGATTCCGCAGTATAAT pLKO_005 3559 CDS 100% 15.000 21.000 N Brwd3 n/a
3 TRCN0000359014 TGATAGATTCCGCAGTATAAT pLKO_005 3559 CDS 100% 15.000 21.000 N BRWD3 n/a
4 TRCN0000364192 TCGAAGACATAGTAGTCAAAT pLKO_005 2500 CDS 100% 13.200 18.480 N BRWD3 n/a
5 TRCN0000160865 GCACGTATTGCAGATGATGAA pLKO.1 5582 CDS 100% 4.950 6.930 N BRWD3 n/a
6 TRCN0000432687 ACAAGACCTGAGACTAATTAA pLKO_005 2377 CDS 100% 15.000 10.500 N BRWD3 n/a
7 TRCN0000418715 TCAAATAAGCCTTACTCATTA pLKO_005 5992 3UTR 100% 13.200 9.240 N BRWD3 n/a
8 TRCN0000161252 GCAGGGAATTTACCAGGAAAT pLKO.1 5863 3UTR 100% 10.800 7.560 N BRWD3 n/a
9 TRCN0000136866 CCCAGGGAAAGCCAAATCATT pLKO.1 5011 CDS 100% 5.625 3.938 N BRWD3 n/a
10 TRCN0000159686 GCCAGTATACATTTGTTTCAA pLKO.1 5944 3UTR 100% 5.625 3.938 N BRWD3 n/a
11 TRCN0000159299 GACAACAAGATTCATCTCTTT pLKO.1 4461 CDS 100% 4.950 3.465 N BRWD3 n/a
12 TRCN0000159352 GCAACAAGAATGGAAGAGTAT pLKO.1 1588 CDS 100% 4.950 3.465 N BRWD3 n/a
13 TRCN0000136128 GAAAGCAACATGGCAATGGAA pLKO.1 5370 CDS 100% 3.000 2.100 N BRWD3 n/a
14 TRCN0000162485 CCACACACACACAAACACATA pLKO.1 6038 3UTR 100% 4.950 2.475 Y BRWD3 n/a
15 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 10055 3UTR 100% 5.625 2.813 Y KLHL30 n/a
16 TRCN0000178741 CACACACATACACACACACAA pLKO.1 6059 3UTR 100% 4.950 2.475 Y Cstad n/a
17 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 10055 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_153252.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

No results found.