Transcript: Mouse NM_153552.3

Mus musculus THO complex 1 (Thoc1), mRNA.

Source:
NCBI, updated 2017-05-28
Taxon:
Mus musculus (mouse)
Gene:
Thoc1 (225160)
Length:
3855
CDS:
14..1987

Additional Resources:

NCBI RefSeq record:
NM_153552.3
NBCI Gene record:
Thoc1 (225160)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_153552.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000123844 GCACTGTTTCATTCCATGTTT pLKO.1 3171 3UTR 100% 5.625 7.875 N Thoc1 n/a
2 TRCN0000123847 CCCAGTGCAATGCTATGAGAA pLKO.1 754 CDS 100% 4.950 6.930 N Thoc1 n/a
3 TRCN0000287401 CCCAGTGCAATGCTATGAGAA pLKO_005 754 CDS 100% 4.950 6.930 N Thoc1 n/a
4 TRCN0000272561 CAAAGATGGTAGAGCATATAT pLKO_005 1137 CDS 100% 15.000 10.500 N THOC1 n/a
5 TRCN0000123848 GCTCCTTACCTGGAAATTAAA pLKO.1 1778 CDS 100% 15.000 10.500 N Thoc1 n/a
6 TRCN0000287403 GCTCCTTACCTGGAAATTAAA pLKO_005 1778 CDS 100% 15.000 10.500 N Thoc1 n/a
7 TRCN0000294945 GTGAATATAAAGCCGTAAATA pLKO_005 1458 CDS 100% 15.000 10.500 N Thoc1 n/a
8 TRCN0000123845 GCACTAAACAAGTCTGGATTA pLKO.1 1919 CDS 100% 10.800 6.480 N Thoc1 n/a
9 TRCN0000287402 GCACTAAACAAGTCTGGATTA pLKO_005 1919 CDS 100% 10.800 6.480 N Thoc1 n/a
10 TRCN0000123846 CGAGGAAGATAATGATGCTTT pLKO.1 1654 CDS 100% 4.950 2.970 N Thoc1 n/a
11 TRCN0000000032 GTATGTGCAATGATCTCCTAA pLKO.1 426 CDS 100% 4.950 3.465 N THOC1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_153552.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07525 pDONR223 100% 90.7% 96.1% None (many diffs) n/a
2 ccsbBroad304_07525 pLX_304 0% 90.7% 96.1% V5 (many diffs) n/a
3 TRCN0000470869 TTTATGCTAAAACAACGGGCGGCC pLX_317 10.6% 90.7% 96.1% V5 (many diffs) n/a
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