Transcript: Human XM_005259573.5

PREDICTED: Homo sapiens fizzy and cell division cycle 20 related 1 (FZR1), transcript variant X3, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
FZR1 (51343)
Length:
3095
CDS:
247..1737

Additional Resources:

NCBI RefSeq record:
XM_005259573.5
NBCI Gene record:
FZR1 (51343)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005259573.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000231905 GGATCCTGGAGTCTCATTAAA pLKO_005 1873 3UTR 100% 15.000 21.000 N FZR1 n/a
2 TRCN0000231903 TGAGGTTCTGGAACGTCTTTA pLKO_005 1646 CDS 100% 13.200 18.480 N FZR1 n/a
3 TRCN0000010856 CGGCAACGATGTGTCTCCCTA pLKO.1 669 CDS 100% 0.880 1.232 N FZR1 n/a
4 TRCN0000231904 CTTCACCAGGATCCGGTAAAC pLKO_005 1719 CDS 100% 10.800 8.640 N FZR1 n/a
5 TRCN0000231901 GTGAACTTCCACAGGATTAAC pLKO_005 421 CDS 100% 13.200 9.240 N FZR1 n/a
6 TRCN0000231902 AGAAGGGTCTGTTCACGTATT pLKO_005 617 CDS 100% 10.800 7.560 N FZR1 n/a
7 TRCN0000004163 GTCTGTTCACGTATTCCCTTA pLKO.1 623 CDS 100% 4.050 2.835 N FZR1 n/a
8 TRCN0000010857 CCAGTCAGAACCGGAAAGCCA pLKO.1 458 CDS 100% 0.250 0.175 N FZR1 n/a
9 TRCN0000010855 CAACGACAACAAGCTGCTGGT pLKO.1 1242 CDS 100% 2.160 1.296 N FZR1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005259573.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03287 pDONR223 100% 99.3% 99.3% None 1440_1448delGGTAAAGTG n/a
2 ccsbBroad304_03287 pLX_304 0% 99.3% 99.3% V5 1440_1448delGGTAAAGTG n/a
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