Construct: ORF ccsbBroad304_03287
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012007.1_s304c1, BRDN0000410155
- Derived from:
- ccsbBroadEn_03287
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FZR1 (51343)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_03287
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51343 | FZR1 | fizzy and cell division cyc... | NM_016263.4 | 100% | 100% | |
| 2 | human | 51343 | FZR1 | fizzy and cell division cyc... | NM_001136198.1 | 99.3% | 99.3% | 1440_1448delGGTAAAGTG |
| 3 | human | 51343 | FZR1 | fizzy and cell division cyc... | XM_005259573.5 | 99.3% | 99.3% | 1440_1448delGGTAAAGTG |
| 4 | human | 51343 | FZR1 | fizzy and cell division cyc... | XM_017026863.2 | 93.3% | 93.3% | 1_96del;1536_1544delGGTAAAGTG |
| 5 | human | 51343 | FZR1 | fizzy and cell division cyc... | NM_001136197.1 | 81.9% | 81.9% | 386_387ins267 |
| 6 | human | 51343 | FZR1 | fizzy and cell division cyc... | XR_001753699.1 | 52.8% | 1_34del;1474_1482delGGTAAAGTG;1523_2797del | |
| 7 | mouse | 56371 | Fzr1 | fizzy/cell division cycle 2... | NM_019757.1 | 88% | 98.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1545
- ORF length:
- 1479
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga ccaggactat gagcggcgcc tgcttcgcca gatcgtcatc cagaatgaga 121 acacgatgcc acgcgtcaca gagatgcggc ggaccctgac gcctgccagc tccccagtgt 181 cctcgcccag caagcacgga gaccgcttca tcccctccag agccggagcc aactggagcg 241 tgaacttcca caggattaac gagaatgaga agtctcccag tcagaaccgg aaagccaagg 301 acgccacctc agacaacggc aaagacggcc tggcctactc tgccctgctc aagaatgagc 361 tgctgggtgc cggcatcgag aaggtgcagg acccgcagac tgaggaccgc aggctgcagc 421 cctccacgcc tgagaagaag ggtctgttca cgtattccct tagcaccaag cgctccagcc 481 ccgatgacgg caacgatgtg tctccctact ccctgtctcc cgtcagcaac aagagccaga 541 agctgctccg gtccccccgg aaacccaccc gcaagatctc caagatcccc ttcaaggtgc 601 tggacgcgcc cgagctgcag gacgacttct acctcaatct ggtggactgg tcgtccctca 661 atgtgctcag cgtggggcta ggcacctgcg tgtacctgtg gagtgcctgt accagccagg 721 tgacgcggct ctgtgacctc tcagtggaag gggactcagt gacctccgtg ggctggtctg 781 agcgggggaa cctggtggcg gtgggcacac acaagggctt cgtgcagatc tgggacgcag 841 ccgcagggaa gaagctgtcc atgttggagg gccacacggc acgcgtcggg gcgctggcct 901 ggaatgctga gcagctgtcg tccgggagcc gcgaccgcat gatcctgcag agggacatcc 961 gcaccccgcc actgcagtcg gagcggcggc tgcagggcca ccggcaggag gtgtgcgggc 1021 tcaagtggtc cacagaccac cagctcctcg cctcgggggg caacgacaac aagctgctgg 1081 tctggaatca ctcgagcctg agccccgtgc agcagtacac ggagcacctg gcggccgtga 1141 aggccatcgc ctggtcccca catcagcacg ggctgctggc ctcggggggc ggcacagctg 1201 accgctgtat ccgcttctgg aacacgctga caggacaacc actgcagtgt atcgacacgg 1261 gctcccaagt gtgcaatctg gcctggtcca agcacgccaa cgagctggtg agcacgcacg 1321 gctactcaca gaaccagatc cttgtctgga agtacccctc cctgacccag gtggccaagc 1381 tgaccgggca ctcctaccgc gtgctgtacc tggcaatgtc ccctgatggg gaggccatcg 1441 tcactggtgc tggagacgag accctgaggt tctggaacgt ctttagcaaa acccgttcga 1501 caaaggagtc tgtgtctgtg ctcaacctct tcaccaggat ccggtaccca actttcttgt 1561 acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat 1621 gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a