Transcript: Human XM_005270737.3

PREDICTED: Homo sapiens membrane associated guanylate kinase, WW and PDZ domain containing 3 (MAGI3), transcript variant X3, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
MAGI3 (260425)
Length:
6627
CDS:
182..3559

Additional Resources:

NCBI RefSeq record:
XM_005270737.3
NBCI Gene record:
MAGI3 (260425)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001147702 AATGGACAGTCATTAACCAA pXPR_003 GGG 1559 46% 10 0.6634 MAGI3 MAGI3 76550
2 BRDN0001148697 AAATGATACTGGATAGTCAG pXPR_003 TGG 1821 54% 10 0.5622 MAGI3 MAGI3 76551
3 BRDN0001145219 CCTGACCTGATAATGCTCGG pXPR_003 TGG 2076 61% 12 0.026 MAGI3 MAGI3 76549
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005270737.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000037865 CCAGGAGTTATTCCTCATAAA pLKO.1 2804 CDS 100% 13.200 9.240 N MAGI3 n/a
2 TRCN0000333008 CCAGGAGTTATTCCTCATAAA pLKO_005 2804 CDS 100% 13.200 9.240 N MAGI3 n/a
3 TRCN0000037864 CCTCACTTTATGTCGTGGTTA pLKO.1 1642 CDS 100% 4.950 3.465 N MAGI3 n/a
4 TRCN0000037867 GCAATGGTTCTGGAGCAGAAT pLKO.1 1784 CDS 100% 4.950 3.465 N MAGI3 n/a
5 TRCN0000333006 GCAATGGTTCTGGAGCAGAAT pLKO_005 1784 CDS 100% 4.950 3.465 N MAGI3 n/a
6 TRCN0000037868 CCCTCAGTATGGGACATACTA pLKO.1 1231 CDS 100% 0.563 0.394 N MAGI3 n/a
7 TRCN0000332940 CCCTCAGTATGGGACATACTA pLKO_005 1231 CDS 100% 0.563 0.394 N MAGI3 n/a
8 TRCN0000037866 GCCATCAAAGATGGCAGAATT pLKO.1 3347 CDS 100% 0.000 0.000 N MAGI3 n/a
9 TRCN0000332945 GCCATCAAAGATGGCAGAATT pLKO_005 3347 CDS 100% 0.000 0.000 N MAGI3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005270737.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

No results found.