Transcript: Human XM_011519956.2

PREDICTED: Homo sapiens sirtuin 3 (SIRT3), transcript variant X4, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
SIRT3 (23410)
Length:
3459
CDS:
1361..2188

Additional Resources:

NCBI RefSeq record:
XM_011519956.2
NBCI Gene record:
SIRT3 (23410)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011519956.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000038892 GTGGGTGCTTCAAGTGTTGTT pLKO.1 1250 5UTR 100% 4.950 6.930 N SIRT3 n/a
2 TRCN0000298766 GTGGGTGCTTCAAGTGTTGTT pLKO_005 1250 5UTR 100% 4.950 6.930 N SIRT3 n/a
3 TRCN0000038890 GCGGCTCTACACGCAGAACAT pLKO.1 1603 CDS 100% 1.650 2.310 N SIRT3 n/a
4 TRCN0000298768 GCGGCTCTACACGCAGAACAT pLKO_005 1603 CDS 100% 1.650 2.310 N SIRT3 n/a
5 TRCN0000038889 CCCAACGTCACTCACTACTTT pLKO.1 1550 CDS 100% 5.625 3.938 N SIRT3 n/a
6 TRCN0000298713 CCCAACGTCACTCACTACTTT pLKO_005 1550 CDS 100% 5.625 3.938 N SIRT3 n/a
7 TRCN0000038891 TCTTGCTGCATGTGGTTGATT pLKO.1 1839 CDS 100% 5.625 3.938 N SIRT3 n/a
8 TRCN0000298765 TCTTGCTGCATGTGGTTGATT pLKO_005 1839 CDS 100% 5.625 3.938 N SIRT3 n/a
9 TRCN0000038893 CCACCTGCACAGTCTGCCAAA pLKO.1 1695 CDS 100% 1.350 0.810 N SIRT3 n/a
10 TRCN0000331109 CCACCTGCACAGTCTGCCAAA pLKO_005 1695 CDS 100% 1.350 0.810 N SIRT3 n/a
11 TRCN0000073413 CGCCTGTAATTCCAGCACTTT pLKO.1 260 5UTR 100% 4.950 2.475 Y LILRB1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011519956.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02761 pDONR223 98.6% 61.6% 61.6% None 0_1ins426;753_806del n/a
2 ccsbBroad304_02761 pLX_304 0% 61.6% 61.6% V5 0_1ins426;753_806del n/a
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