Construct: ORF ccsbBroad304_02761
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000874.1_s304c1, BRDN0000402401
- Derived from:
- ccsbBroadEn_02761
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SIRT3 (23410)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02761
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23410 | SIRT3 | sirtuin 3 | NM_012239.6 | 100% | 100% | |
| 2 | human | 23410 | SIRT3 | sirtuin 3 | NM_001370310.1 | 95.6% | 95.6% | 1179_1232del |
| 3 | human | 23410 | SIRT3 | sirtuin 3 | NM_001370314.1 | 86.4% | 86.4% | 806_807ins162 |
| 4 | human | 23410 | SIRT3 | sirtuin 3 | NM_001370312.1 | 80.3% | 80.3% | 279_280ins192;987_1040del |
| 5 | human | 23410 | SIRT3 | sirtuin 3 | NM_001370315.1 | 79.6% | 79.6% | 135_136ins243 |
| 6 | human | 23410 | SIRT3 | sirtuin 3 | NM_001017524.2 | 64.4% | 64.4% | 0_1ins426 |
| 7 | human | 23410 | SIRT3 | sirtuin 3 | NM_001370318.1 | 64.4% | 64.4% | 0_1ins426 |
| 8 | human | 23410 | SIRT3 | sirtuin 3 | NM_001370319.1 | 64.4% | 64.4% | 0_1ins426 |
| 9 | human | 23410 | SIRT3 | sirtuin 3 | NM_001370320.1 | 64.4% | 64.4% | 0_1ins426 |
| 10 | human | 23410 | SIRT3 | sirtuin 3 | XM_024448410.1 | 64.4% | 64.4% | 0_1ins426 |
| 11 | human | 23410 | SIRT3 | sirtuin 3 | NM_001370321.1 | 61.6% | 61.6% | 0_1ins426;753_806del |
| 12 | human | 23410 | SIRT3 | sirtuin 3 | NM_001370322.1 | 61.6% | 61.6% | 0_1ins426;753_806del |
| 13 | human | 23410 | SIRT3 | sirtuin 3 | NM_001370323.1 | 61.6% | 61.6% | 0_1ins426;753_806del |
| 14 | human | 23410 | SIRT3 | sirtuin 3 | XM_011519956.2 | 61.6% | 61.6% | 0_1ins426;753_806del |
| 15 | human | 23410 | SIRT3 | sirtuin 3 | XM_011519957.2 | 61.6% | 61.6% | 0_1ins426;753_806del |
| 16 | human | 23410 | SIRT3 | sirtuin 3 | XM_017017431.1 | 61.6% | 61.6% | 0_1ins426;753_806del |
| 17 | human | 23410 | SIRT3 | sirtuin 3 | NM_001370324.1 | 46.2% | 45.5% | (many diffs) |
| 18 | human | 23410 | SIRT3 | sirtuin 3 | NM_001370325.1 | 46.2% | 45.5% | (many diffs) |
| 19 | human | 23410 | SIRT3 | sirtuin 3 | NM_001370316.1 | 43.8% | 43.8% | 135_136ins672 |
| 20 | human | 23410 | SIRT3 | sirtuin 3 | NR_163396.1 | 40.2% | 1_14del;719_807del;1301_2971del | |
| 21 | human | 23410 | SIRT3 | sirtuin 3 | NR_163399.1 | 36.3% | 1_14del;820_1228del;1621_3291del | |
| 22 | human | 23410 | SIRT3 | sirtuin 3 | NR_163397.1 | 35.9% | (many diffs) | |
| 23 | human | 23410 | SIRT3 | sirtuin 3 | NR_163387.1 | 35.5% | (many diffs) | |
| 24 | human | 23410 | SIRT3 | sirtuin 3 | NR_163401.1 | 35.4% | (many diffs) | |
| 25 | human | 23410 | SIRT3 | sirtuin 3 | NR_163400.1 | 35.3% | (many diffs) | |
| 26 | human | 23410 | SIRT3 | sirtuin 3 | NR_163388.1 | 34.9% | (many diffs) | |
| 27 | human | 23410 | SIRT3 | sirtuin 3 | NR_163394.1 | 34.6% | (many diffs) | |
| 28 | human | 23410 | SIRT3 | sirtuin 3 | NR_163390.1 | 34.3% | (many diffs) | |
| 29 | human | 23410 | SIRT3 | sirtuin 3 | NR_163386.1 | 34.2% | (many diffs) | |
| 30 | human | 23410 | SIRT3 | sirtuin 3 | NR_163402.1 | 34.2% | (many diffs) | |
| 31 | human | 23410 | SIRT3 | sirtuin 3 | NR_163398.1 | 34% | (many diffs) | |
| 32 | human | 23410 | SIRT3 | sirtuin 3 | NR_163395.1 | 31.9% | (many diffs) | |
| 33 | human | 23410 | SIRT3 | sirtuin 3 | NM_001370317.1 | 31.8% | 31.8% | 0_1ins816 |
| 34 | human | 23410 | SIRT3 | sirtuin 3 | NR_163389.1 | 31.6% | (many diffs) | |
| 35 | human | 23410 | SIRT3 | sirtuin 3 | NR_163392.1 | 30.8% | (many diffs) | |
| 36 | human | 23410 | SIRT3 | sirtuin 3 | NR_163393.1 | 30.3% | (many diffs) | |
| 37 | human | 23410 | SIRT3 | sirtuin 3 | NR_163391.1 | 30.2% | (many diffs) | |
| 38 | mouse | 64384 | Sirt3 | sirtuin 3 | NM_001127351.1 | 54% | 55.1% | (many diffs) |
| 39 | mouse | 64384 | Sirt3 | sirtuin 3 | NM_022433.2 | 54% | 55.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1263
- ORF length:
- 1197
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc gttctggggt tggcgcgccg cggcagccct ccggctgtgg ggccgggtag 121 ttgaacgggt cgaggccggg ggaggcgtgg ggccgtttca ggcctgcggc tgtcggctgg 181 tgcttggcgg cagggacgat gtgagtgcgg ggctgagagg cagccatggg gcccgcggtg 241 agcccttgga cccggcgcgc cccttgcaga ggcctcccag acccgaggtg cccagggcat 301 tccggaggca gccgagggca gcagctccca gtttcttctt ttcgagtatt aaaggtggaa 361 gaaggtccat atctttttct gtgggtgctt caagtgttgt tggaagtgga ggcagcagtg 421 acaaggggaa gctttccctg caggatgtag ctgagctgat tcgggccaga gcctgccaga 481 gggtggtggt catggtgggg gccggcatca gcacacccag tggcattcca gacttcagat 541 cgccggggag tggcctgtac agcaacctcc agcagtacga tctcccgtac cccgaggcca 601 tttttgaact cccattcttc tttcacaacc ccaagccctt tttcactttg gccaaggagc 661 tgtaccctgg aaactacaag cccaacgtca ctcactactt tctccggctg cttcatgaca 721 aggggctgct tctgcggctc tacacgcaga acatcgatgg gcttgagaga gtgtcgggca 781 tccctgcctc aaagctggtt gaagctcatg gaacctttgc ctctgccacc tgcacagtct 841 gccaaagacc cttcccaggg gaggacattc gggctgacgt gatggcagac agggttcccc 901 gctgcccggt ctgcaccggc gttgtgaagc ccgacattgt gttctttggg gagccgctgc 961 cccagaggtt cttgctgcat gtggttgatt tccccatggc agatctgctg ctcatccttg 1021 ggacctccct ggaggtggag ccttttgcca gcttgaccga ggccgtgcgg agctcagttc 1081 cccgactgct catcaaccgg gacttggtgg ggcccttggc ttggcatcct cgcagcaggg 1141 acgtggccca gctgggggac gtggttcacg gcgtggaaag cctagtggag cttctgggct 1201 ggacagaaga gatgcgggac cttgtgcagc gggaaactgg gaagcttgat ggaccagaca 1261 aatacccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc 1321 tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc 1381 tctggatta