Transcript: Human XM_011535833.2

PREDICTED: Homo sapiens mannosidase alpha class 1A member 1 (MAN1A1), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
MAN1A1 (4121)
Length:
4076
CDS:
67..1461

Additional Resources:

NCBI RefSeq record:
XM_011535833.2
NBCI Gene record:
MAN1A1 (4121)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011535833.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000378329 TCTAGCAGCGGACTAACTTAT pLKO_005 853 CDS 100% 13.200 18.480 N MAN1A1 n/a
2 TRCN0000049600 CCTTTATCCTAACTATCTGAA pLKO.1 663 CDS 100% 4.950 6.930 N MAN1A1 n/a
3 TRCN0000049598 CGAGACTCATTTGATCCGCAA pLKO.1 831 CDS 100% 2.160 3.024 N MAN1A1 n/a
4 TRCN0000359712 CCCTTCACTGTATACCTTAAT pLKO_005 1493 3UTR 100% 13.200 9.240 N MAN1A1 n/a
5 TRCN0000359711 GACCTAAATTCCTTATCATAT pLKO_005 1623 3UTR 100% 13.200 9.240 N MAN1A1 n/a
6 TRCN0000359778 GACTACTCTCAGCCTACTATC pLKO_005 362 CDS 100% 10.800 7.560 N MAN1A1 n/a
7 TRCN0000049599 GCAGAGTGAATGGAGGCTATT pLKO.1 1235 CDS 100% 10.800 7.560 N MAN1A1 n/a
8 TRCN0000055383 CGAAAGAAAGCAGTGGAACTT pLKO.1 403 CDS 100% 4.950 3.465 N Man1a n/a
9 TRCN0000049602 GTAACATCAAAGGAGCAACTA pLKO.1 200 CDS 100% 4.950 3.465 N MAN1A1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011535833.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10957 pDONR223 100% 21.8% 19.8% None (many diffs) n/a
2 ccsbBroad304_10957 pLX_304 0% 21.8% 19.8% V5 (many diffs) n/a
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