Construct: ORF ccsbBroad304_10957
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010493.1_s304c1, BRDN0000461003
- Derived from:
- ccsbBroadEn_10957
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAN1A1 (4121)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10957
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4121 | MAN1A1 | mannosidase alpha class 1A ... | XM_011535834.3 | 76.4% | 76.4% | 1_249del;850_851ins69 |
| 2 | human | 4121 | MAN1A1 | mannosidase alpha class 1A ... | NM_005907.4 | 50.2% | 49.2% | (many diffs) |
| 3 | human | 4121 | MAN1A1 | mannosidase alpha class 1A ... | XM_005266986.4 | 44.7% | 43.8% | (many diffs) |
| 4 | human | 4121 | MAN1A1 | mannosidase alpha class 1A ... | XM_011535833.2 | 21.8% | 19.8% | (many diffs) |
| 5 | human | 4121 | MAN1A1 | mannosidase alpha class 1A ... | XR_001743420.1 | 20.2% | (many diffs) | |
| 6 | mouse | 17155 | Man1a | mannosidase 1, alpha | NM_008548.4 | 45% | 44.5% | (many diffs) |
| 7 | mouse | 17155 | Man1a | mannosidase 1, alpha | XM_006512569.3 | 45% | 44.5% | (many diffs) |
| 8 | mouse | 17155 | Man1a | mannosidase 1, alpha | XM_006512568.2 | 39.7% | 39.2% | (many diffs) |
| 9 | mouse | 17155 | Man1a | mannosidase 1, alpha | XR_001779471.1 | 17.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1167
- ORF length:
- 1101
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgcc cgtggggggc ctgttgccgc tcttcagcag ccccgcgggc ggcgtcctgg 121 gcggggggct cggcggcggc ggtggcagga aggggtcggg ccccgccgcc ctccgcctga 181 cggagaagtt cgtgctgctg ctggtattca gcgccttcat cacgctctgc ttcggggcga 241 tcttcttcct gccagactcc tccaagctgc tcagcggggt cctgttccac tccagccccg 301 ccttgcagcc ggccgccgac cacaagcccg ggcccggggc gcgcgccgag gacgcggccg 361 aggggcgagc ccggcgccgc gaggaggggg cacccgggga cccggaggcc gccctggagg 421 acaacttggc caggatccgc gaaaaccacg agcgggctct cagggaagcc aaggagaccc 481 tgcagaagct gcccgaggag atccaaagag acatcctact ggagaagaag aaggtggccc 541 aggaccagct gcgtgacaag gcgccgttca gaggcctgcc cccggtggac ttcgtgcccc 601 caatcggggt ggagagccgg gagcccgccg acgccgccat ccgcgagaaa agggcaaaga 661 tcaaagagga aattgaagcc aggaaggggc aagcaaattg cccagggtcg agcagctcct 721 gcgaggttga aatccagatg atgaaacatg cttggaataa ttataaaggt tatgcctggg 781 gattaaatga actcaaacct atatcaaaag gaggccattc aagcagtttg tttggtaaca 841 tcaaaggagc aactatagta gatgccctgg atacactttt tattatggaa atgaaacatg 901 aatttgaaga agcaaaatca tgggttgaag aaaatttaga ttttaatgtg aatgctgaaa 961 tttctgtctt tgaagtaaat atacgctttg ttggtggact actctcagcc tactatctgt 1021 ctggagaaga gatttttcga aagaaagcag tggaacttgg ggtaaaattg ctacctgcat 1081 ttcatactcc ctctggaata ccttgggcat tgctgaatat gaaaaggtgg atgtgcaata 1141 tcccaagggc tatggaggct accatatgcc caactttctt gtacaaagtg gttggtaagc 1201 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt 1261 ggcgcgttaa gtcgacaatc aacctctgga tta